世界中医药

作者	单位
江一静1,2，林志诚1，郑美1，游咏梅1，薛偕华1，夏敏1，詹增土1,2	1 福建中医药大学附属康复医院,福州,350003 2 福建省康复技术重点实验室,福州,350003

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中文摘要:

目的:观察针刺曲池穴及足三里穴对MCAO模型大鼠行为学、超微结构及TGF-β1(transforming growth factor-β1)的影响,探析针刺的神经保护机制。方法:将60只健康成年的Sprague Dawley大鼠(SD鼠)常规驯养3 d后随机分为假手术组、模型组和针刺组,各20只。模型组及针刺组大鼠接受线栓法进行脑缺血模型制备,假手术组大鼠仅接受皮肤切开及血管剥离术,假手术组及模型组术后回笼继续饲养,每日模拟捉拿、穴位点触一次。针刺组接受针刺曲池穴及足三里穴,1次/d,30 min/次,连续干预7 d。治疗前后记录大鼠catwalk系统动静态指标的变化,TTC染色观察大鼠脑梗死体积的变化,透射电镜观察大鼠脑组织超微结构的变化,Western blot检测各组大鼠脑组织TGF-β1浓度的差异。结果:1)Catwalk系统参数分析:造模组大鼠步行速度明显慢于假手术组,步行时间明显较针刺组长,其中针刺组优于模型组,差异均有统计学意义(P<0.05);2)TTC及其图像软件分析:造模组大鼠存在明显脑梗死区域,针刺组脑梗死体积小于模型组(P<0.05);3)透射电镜:假手术组大鼠脑组织神经元细胞结构基本正常,模型组神经元细胞结构受破坏,包括细胞膜不完整、细胞核肿胀、内质网减少等,与模型组比较,针刺组大鼠脑组织具有较为完整的细胞膜及细胞核结构,线粒体空泡病变现象亦有明显改善。4)Western blot:假手术大鼠脑组织的TGF-β1浓度明显高于2组造模大鼠,经过针刺干预后2组造模大鼠脑组织TGF-β1均高于假手术组,其中针刺组高于模型组,差异有统计学意义(P<0.05)。结论:针刺曲池穴及足三里穴可通过上调TGF-β1浓度实现神经细胞增殖及结构修复,从而发挥神经保护作用。

English Summary:

To observe the effects of acupuncture at Quchi (LI 11) and Zusanli (ST 36) on the behavior, ultrastructure and protein TGF-β1 (transforming growth factor beta 1) in the MCAO model rats, and to explore and analyze the neuroprotective mechanism of acupuncture. Methods:After 3 days' conventional domestication, 60 healthy adult Sprague Dawley (SD) rats were randomly divided into the control group, model group and acupuncture group, with 20 in each group. The rats in the both model group and acupuncture group accepted middle cerebral artery occlusion method for cerebral ischemia model preparation, and rats of control group received only the skin incision and vascular decollement. The postoperative control group and model group continue feeding in the cages, simulation captured once daily. Acupuncture group received acupuncture at LI 11 and ST 36, 1 time/day, 30 min/time, continuous intervention for 7 days. Rats' dynamic and static index changes in the catwalk system before and after treatment were recorded. TTC staining was used to observe rat infarction volume changes. The ultrastructure changes of the rats were observed by transmission electron microscope (TEM). The concentration differences of TGF-β1 were detected by Western blot in the groups of rats. Results:1) The Catwalk system parameter analysis:the walking speed of rats in the model groups was significantly slower than the control group, and the walking time longer than the acupuncture group. The acupuncture group was better than the model group, and the differences were with statistically significance (P<0.05); 2) The TTC and image software analysis:the rats of molding groups had obvious cerebral infarction area, and the cerebral infarction volume of acupuncture group was less than the model group (P<0.05); 3) Transmission electron microscopy (TEM) :the neurons structure in the rats of control group was basically normal, and destroyed in the model group, including the incomplete cytomembrane, cell nucleus swelled, endoplasmic reticulum decreased. Compared with model group, the rats of acupuncture group had a relatively complete cytomembrane and nucleus structure, and mitochondria vacuoles had also improved significantly. 4) Western blot:after intervention, TGF-β1 in the brain of the two molding groups was higher than that in control group. The acupuncture group was higher than the control group, and the differences were with statistically significance (P<0.05). Conclusion:Acupuncture at Quchi (LI 11) and Zusanli (ST 36) can achieve neurocyte proliferation and structure repair by up-regulating the concentration of TGF-β1, so as to play a role of neuroprotection.

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