世界中医药

目的:探究姜黄素对人胰腺癌SW1990细胞增殖抑制作用及对Wnt信号通路的影响。方法:低/中/高剂量观察组人胰腺癌SW1990细胞分别以终浓度为20、50、100 μmol/L姜黄素处理,100 μL/孔,对照组细胞则以等体积生理盐水处理,分别干预24、48、72 h。以噻唑蓝(MTT)法及Annexin-V/FITC双染法分别检测人胰腺癌细胞SW1990增殖及凋亡情况,反转录聚合酶链式反应(RT-PCR)法及细胞爬片免疫细胞化学技术检测人胰腺癌细胞SW1990 β-链蛋白(β-Catenin) mRNA及β-Catenin蛋白表达情况。结果:干预72 h后低/中/高剂量观察组人胰腺癌SW1990细胞增殖抑制率(%)分别为(4.59±1.32)、(40.01±3.87)、(60.98±5.97);对照组及低/中/高剂量观察组人胰腺癌SW1990细胞凋亡率(%)（8.14±1.25）、（13.04±1.03）、（27.15±1.62）、(59.21±1.46);β-Catenin蛋白免疫细胞化学(ICC)评分(分)分别为(5.78±0.56)、(4.42±0.61)、(2.57±0.58)、(1.38±0.63)。观察组凋亡率显著高于对照组,β-Catenin mRNA相对表达量及β-Catenin蛋白ICC评分显著低于对照组(P<0.05),且增殖抑制率、凋亡率随姜黄素作用浓度增加逐渐升高(P<0.05),增殖抑制率亦随干预时间延长逐渐升高(P<0.05);β-Catenin mRNA相对表达量及β-Catenin蛋白ICC评分随姜黄素作用浓度增加逐渐降低(P<0.05)。结论:姜黄素可有效下调人胰腺癌细胞SW1990 β-Catenin基因表达,抑制Wnt信号通路活化,具有显著的增殖抑制作用,且具有明显的时间-剂量依赖性。

English Summary:

To explore the proliferation inhibition effect of curcumin on human pancreatic cancer SW1990 cell and the influence on Wnt signaling pathway. Methods:The human pancreatic cancer cells SW1990 in experimental-L/M/H group were treated with final concentration of 20, 50, 100 μmol/L curcumin, 100 μL/hole, and cells in control group were treated with isovolumetric saline. Each group was intervened for 24, 48, 72 h respectively. The proliferation and apoptosis were detected by methyl thiazolyl tetrazolium (MTT) method and Annexin-V/FITC double staining respectively, expression of β-Catenin mRNA and β-Catenin mRNA protein in human pancreatic cancer SW1990 cell were detected by reverse transcription PCR (RT-PCR) method and cell climbing immunocytochemistry technique respectively. Results:72 h after intervened, the proliferation inhibition rates (%) of human pancreatic cancer SW1990 cell in experimental-L/M/H group were 4.59±1.32, 40.01±3.87, 60.98±5.97 respectively; Apoptosis rates (%) in control group and experimental-L/M/H group were 8.14±1.25, 13.04±1.03, 27.15±1.62, 59.21±1.46 respectively; β-Catenin protein immunocytochemistry (ICC) score (score) were 5.78±0.56, 4.42±0.61, 2.57±0.58, 1.38±0.63 respectively. The apoptosis rate in experimental groups were higher than that in control, and β-Catenin mRNA relative expression and β-Catenin protein ICC score were lower than those in control group (P<0.05), and the proliferation inhibition rate, apoptosis rate in experimental groups increased gradually with the increase of curcumin action concentration (P<0.05). Proliferation inhibition also increased gradually with the lengthen of action time (P<0.05); the β-Catenin mRNA relative expression and β-Catenin protein ICC score decreased gradually with the increase of curcumin action concentration (P<0.05). Conclusion:Curcumin can down-regulate the expression of β-Catenin in human pancreatic cancer SW1990 cell effectively, inhibit the Wnt signaling pathway excitation, and has significant proliferation inhibition effect, which has an obvious time-dose dependence.

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