引用本文:黄英兰,杨芳.基于指纹图谱结合多指标成分定量用于麻黄汤的质量评价[J].世界中医药,2018,(05):. |
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基于指纹图谱结合多指标成分定量用于麻黄汤的质量评价 |
Quantitative Evaluation of Mahuang Decoction Based on Fingerprint and Multi Index Components |
投稿时间:2017-05-08 |
DOI:10.3969/j.issn.1673-7202.2018.05.052 |
中文关键词: 指纹图谱 成分 定量 麻黄汤 质量评价 |
English Keywords:Fingerprint Quantitative analysis Mahuang Decoction Quality evaluation |
基金项目:佛山市科技创新专项基金(2014AG10003) |
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中文摘要: |
目的:分析和评价指纹图谱和多指标成分定量结合用于麻黄汤主要成分的定量和质量。方法:采用线性范围试验和加样回收率实验考察高效液相色谱法(HPLC)指纹图谱分析方法的严谨性,由2个厂家各提供6个不同批次麻黄汤药材,共12批次,以盐酸麻黄碱对照品、桂皮醛对照品、苦杏仁苷对照品、甘草酸对照品,多指标成分定量HPLC测定,分别计算各批次麻黄汤中这4种成分的含量;同时进行指纹图谱HPLC分析评价麻黄汤的质量。结果:麻黄汤中的有效成分盐酸麻黄碱、桂皮醛、苦杏仁苷、甘草酸在各自浓度范围内的线性关系良好,且这4种成分的平均回收率分别是100.0%、100.1%、99.39%、99.04%,RSD分别是1.7%、1.3%、1.1%、0.99%。D厂中的麻黄汤药材样品质量波动较大,相似度较低,而B厂中麻黄汤药材质量波动较小,相似度较高,一致性较好。结论:麻黄汤的主要成分是盐酸麻黄碱、桂皮醛、苦杏仁苷和甘草酸,B厂麻黄汤的药材质量优于D厂。 |
English Summary: |
To analyze the quantitative and qualitative evaluation of the main components of Mahuang Decoction by quantitative analysis of fingerprint and multi index components. Methods:The linear range test and recovery test were used on high performance liquid chromatography (HPLC) analysis method for the rigor of the fingerprint. Two manufacturers provided 6 different batches of Mahuang Decoction, with a total of 12 batches, and with ephedrine hydrochloride, cinnamaldehyde, bitter apricot kernel, licorice glycoside reference acid as control products. Quantitative determination of HPLC content in the 4 components of each batch in Mahuang Decoction was calculated; at the same time, fingerprint HPLC was used to evaluate the quality of Mahuang Decoction. Results:1) In Mahuang Decoction, the effective ingredients including ephedrine, cinnamaldehyde, amygdalin and glycyrrhizic acid had good linear relationship within the scope of concentration was good, and the average recovery rate of 4 components were 100%, 100.1%, 99.39%, 99.04%, with RSD of 1.7%, 1.3%, 1.1%, 0.99%. 2) The quality fluctuation of Mahuang Decoction samples from D manufactures was larger and the similarity was lower, while the quality fluctuation was lower, with high similarity and good consistency in B manufacture. Conclusion:The main components of Mahuang Decoction are ephedrine hydrochloride, cinnamic aldehyde, amygdalin and glycyrrhizic acid. The quality of Mahuang Decoction of B manufacture was better than that of D manufacture. |
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