To observe the changes of behavior and learning memory ability of chronic immobilization stress liver-stagnation and spleen-deficiency syndrome rats and the regulatory effect of Xiaoyao Powder.Methods:A total of 45 male SD rats were randomly divided into 3 groups：normal control group,model group and Xiaoyao powder group.The rat model was established by chronic immobilization stress (daily binding for 3 h,for 21 days) and intervened with Xiaoyao powder.The general state of rats in each group was observed,and the body mass,food intake,open-field experiment behavioral indicators were measured.Y-maze was used to test the learning and memory ability.Results:The rats in the model group showed strong resistance and screaming at the early stage of chronic immobilization stress,and later appeared as burnout,emotional depression and desperate state of behavior of no longer resistance.Compared with normal group：the body mass and food intake of model group rats decreased significantly (P<0.01); In the open-field experiment,the total length of 5 minutes motion decreased (P<0.05),and the average moving speed of 5 minutes slowed down (P<0.05),and the stay period in center zone increased (P<0.05); In Y-maze test,the rats in model group showed that the correct reaction rate decreased (P<0.01),and the total reaction time increased throughout the day (P<0.01).Xiaoyao Powder can regulate the above changes in the model group rats.Conclusion:Xiaoyao Powder can regulate the weight,food intake and abnormal behaviors of liver-qi and spleen deficiency rats.It can also improve the learning and memory ability and the state of depression or anxiety.