世界中医药

作者	单位
张秋玲1，韩超1，徐俊2，杨柳2，梁慧玲1，杨洁颖1	1 广州市中医医院，广州，510130； 2 暨南大学药学院，广州，510632

摘要点击次数: 1215

全文下载次数: 1088

中文摘要:

目的：探讨翘芩清肺剂在改善哮喘气道炎症及其可能的作用机制。方法：选取雌性SD大鼠50只，制备哮喘大鼠模型并随机分成5组，即正常组、模型组、地塞米松组和翘芩清肺剂低、高剂量组。分别采用酶联免疫吸附试验（ELISA）测定支气管肺泡灌洗液（BALF）中白细胞介素-1（IL-1）、IL-5及干扰素-γ（IFN-γ）的含量，并观察肺组织病理学改变；用免疫组织化学染色法观察细胞外信号调节蛋白激酶（ERK）在肺组织内的表达，转化生长因子（TGF）-β1在支气管组织内的表达；用实时定量-聚合酶链反应（Real-time PCR）测定气道平滑肌组织中ERK mRNA含量。结果：模型组中ERK蛋白含量及mRNA水平，TGF-β1蛋白表达较正常组有显著增加（P<0.05）；与模型组比较，各干预组均能明显降低ERK mRNA及TGF-β1蛋白水平（P<0.05）。结论：翘芩清肺剂可明显改善哮喘大鼠的呼吸道症状，对气道具有保护作用，其作用机制可能与调节IL-4/IFN-γ平衡失调、抑制ERK通路的磷酸化，减轻气道炎症有关。

English Summary:

To investigate the Qiaoqin Qingfei Formula in improving airway inflammation and its possible mechanism. Methods:Fifty female SD rats with asthma were randomly divided into 5 groups：normal group, model group, dexamethasone group and, low and high dose group of Qiaoqin Qingfei Formula. The contents of interleukin-1 (IL-1), interleukin-5 (IL-5) and interferon gamma (IFN-γ) in bronchoalveolar lavage fluid (BALF) were detected with ELISA method after administration. The proteins expression of the Erk1/2 of the lung tissues and the TGF-β1 of the bronchial tissues were analyzed by Western blot method and the mRNA expression of ERK1/2 of the bronchial tissues were analyzed by Real-time PCR. Results:Compared with normal group, the mRNA expression of ERK and the protein expression of TGF-β1 were significantly increased (P<0.01). Compared with the model group, all the treatment groups significantly decreased the expression of the ERK mRNA and the protein of TGF-β1 (P<0.05-0.01). Conclusion:Qiaoqin Qingfei Formula can significantly improve the symptoms of respiratory tract which has a protective effect on asthmatic rats. Its mechanism may be related to the inhibition of the phosphorylation of ERK signaling pathway, regulation of IL-4/IFN-γimbalance and reduction of inflammatory cell infiltration.

查看全文查看/发表评论下载PDF阅读器