世界中医药
文章摘要
引用本文:杨桃1,张静1,李丽娜2,鲁艺2,王淑艳2,黄翔2,张波1,畅洪昇1.培元解郁方对急性糖皮质激素诱导SD大鼠的抗抑郁作用及TRY-KYN代谢途径的调节[J].世界中医药,2018,(08):.  
培元解郁方对急性糖皮质激素诱导SD大鼠的抗抑郁作用及TRY-KYN代谢途径的调节
Antidepressant Effect of Peiyuan Jieyu Decoction and Its Regulation on TRY-KYN Metabolic Pathway on Acute glucocorticoid on SD rats
投稿时间:2017-09-09  
DOI:10.3969/j.issn.1673-7202.2018.08.036
中文关键词:  培元解郁方  抗抑郁  色氨酸-犬尿氨酸代谢途径
English Keywords:Peiyuan Jieyu Decoction  Antidepression  TRY-KYN
基金项目:国家自然科学基金项目(81373584)
作者单位
杨桃1,张静1,李丽娜2,鲁艺2,王淑艳2,黄翔2,张波1,畅洪昇1 1 北京中医药大学中药学院北京102488 2 北京中医药大学基础医学院北京100029 
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中文摘要:
      目的:研究培元解郁方的抗抑郁作用以及对色氨酸(TRY)-犬尿氨酸(KYN)代谢的调节机制。方法:建立氢化可的松大鼠抑郁模型,以蔗糖消耗反映大鼠的抑郁状态,采用高效液相-质谱联用(HPLC-MS/MS)技术检测脑组织KYN、TRY、喹啉酸(QUIN)、犬尿烯酸(KA)含量,实时荧光定量PCR技术测定肝组织中色氨酸2,3-双加氧酶(TDO)mRNA表达水平和脑组织海马中吲哚胺2,3-双加氧酶(IDO)、5-HT1A的mRNA表达。结果:造模21 d后,大鼠对蔗糖的偏好降低,KYN/TRY比值升高,QUIN含量升高,KA含量降低,TDO mRNA的表达水平增加,5-HT1A mRNA的表达水平降低,差异有统计学意义(P<0.05)。各给药组能提高大鼠对蔗糖的偏好,差异有统计学意义(P<0.05)。各给药组均能显著降低KYN/TRY比值,差异有统计学意义(P<0.05),培元解郁方中剂量组和天丝饮组能显著提高KA的含量,降低QUIN的含量,差异有统计学意义(P<0.05)。天丝饮能明显降低肝脏中TDO和海马中IDO mRNA的表达,差异有统计学意义(P<0.05)。四逆散和培元解郁方中、高剂量能够增加海马中5-HT1A mRNA的表达,差异有统计学意义(P<0.05)。结论:天丝饮、四逆散和培元解郁方均有抗抑郁药样作用,机制与降低TRY-KYN代谢的关键酶mRNA表达,降低神经毒性代谢产物和增加神经保护性代谢产物,从而提高神经元保护作用,以及增加5-HT1A的mRNA表达有关。
English Summary:
      To study the antidepressant effects of Peiyuan Jieyu Decoction and the regulatory mechanism of the tryptophan-kynurenine (TRY-KYN) metabolic pathway.Methods:The rat model of depression induced by intraperitoneal injection hydrocortisone was built.The symptoms of depression were observed by sucrose preference experiment.The contents of tryptophane,kynurenine,kynurenic acid and quinaldinic acid in brain tissue were detected by HPLC-MS/MS technique.The mRNA expression levels of TDO in liver tissue,IDO and 5-HT1A in brain tissue were detected by using real-time PCR.Results:After 21 days' acute stress,the preference in sucrose was reduced.The ratio of KYN/TRY and the content of QUIN were increased.The content of KA was decreased.The expression of TDO mRNA increased and the expression of 5-HT1A mRNA decreased (P<0.05).The Fluoxetine group,Tiansi decoction group,Sini decoction group,high dose and middle dose of Peiyuan Jieyu decoction group could improve the preference in sucrose preference experiment (P<0.05).Each treatment group could obviously reduce the ratio of KYN/TRY (P<0.05).Tiansi decoction and middle dose of Peiyuan Jieyu decoction could significantly improve the content of KA,and decrease the content of QUIN P<0.05).Tiansi decoction could obviously decrease the expression of TDO mRNA in liver and the expression of IDO mRNA in hippocampus (P<0.05).Sini decoction,middle dose and high dose of Peiyuan Jieyu decoction could increase the expression of 5-HT1A mRNA in hippocampus (P<0.05).Conclusion:Traditional Chinese medicine groups have antidepressant effects.Its mechanism may be connected with reducing the expression of the key enzyme mRNA in TRY-KYN metabolic pathway,improving the neuron protective effect by reducing neurotoxic metabolites and increasing neuroprotective metabolites,and increasing the expression of 5-HT1A.
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