| 引用本文:吴胜斌，王应灯.人参皂苷20（s）-Rg3对llc-pk1细胞中顺铂所致肾毒性的保护作用[J].世界中医药,2018,(08):. |
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| 人参皂苷20（s）-Rg3对llc-pk1细胞中顺铂所致肾毒性的保护作用 |
| Protective Effects of Ginsenoside 20 (s)-Rg3 on Renal Toxicity Induced by Cisplatin in llc-pk1 Cells |
| 投稿时间：2017-11-03 |
| DOI：10.3969/j.issn.1673-7202.2018.08.045 |
| 中文关键词: 顺铂 人参皂苷20（S）-Rg3 llc-pk1细胞 c-Jun N-末端激酶 肾毒性 发酵黑参 丝裂原活化蛋白激酶 细胞外调节蛋白激酶 磷酸脱氢酶 甘油醛-3-磷酸脱氢酶 |
| English Keywords:Cisplatin Ginsenoside 20 (S)-Rg3 llc-pk1 cell c-Jun N-terminal kinase Nephrotoxicity FBG MAPK ERK Phosphate dehydrogenase Glyceraldehyde-3-phosphate dehydrogenase |
| 基金项目:上海市自然科学基金项目(09ZR417400) |
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| 中文摘要: |
| 目的：探讨人参皂苷20（s）-Rg3对llc-pk1细胞中顺铂所致肾毒性的保护作用。方法：通过基于细胞的肾脏保护试验,研究了发酵黑参（FBG）及其活性成分人参皂苷20（S）-Rg3对猪肾（LLC-PK1）细胞中顺铂（化疗药物）诱导的损伤的保护作用。结果：由顺铂诱导的细胞活力降低，再使用FBG提取物和人参皂苷20（S）-Rg3依赖剂量后明显恢复。用FBG提取物或人参皂苷20（S）-Rg3处理后会降低顺铂诱导升高与磷酸化c-Jun N-末端激酶（JNK），p53和裂解的半胱天冬酶-3升高的蛋白。通过用FBG和人参皂苷20（S）-Rg3的共同处理，由于顺铂的诱导导致凋亡LLC-PK1细胞升高的百分比显著降低。结论：人参皂苷20（s）-Rg3可改善LLC-PK1的细胞毒性，人参皂苷20(S)-RG3可能是通过阻断JNK-P53-caspase-3信号级联反应来介导这种作用的重要组成部分。 |
| English Summary: |
| To discuss protective effects of ginsenoside 20 (s)-Rg3 on renal toxicity induced by cisplatin in llc-pk1 cells. Methods:This article studied the protective effect of fermentation of black ginseng cells (FBG) and its active component ginsenoside 20 (S)-Rg3 on porcine kidney (LLC-PK1) cells in cisplatin (chemotherapy)-induced injury based on kidney protection test. Results:The cisplatin induced cell viability decreased, and then FBG was used to extract and ginsenoside 20 (S)-Rg3 was restored by FBG. After the dose dependent or extract ginsenoside 20 (S) after-Rg3 treatment may reduce cisplatin induced increased phosphorylation of c-Jun and N-terminal kinase (JNK), p53 and caspase cleavage the increase of-3 protein. By using FBG and 20 ginsenosides (S) together with-Rg3, the percentage of cisplatin induced apoptosis in LLC-PK1 cells leaded to increased significantly. Conclusion:FBG and its major ginsenoside 20(S)-Rg3, ameliorated cisplatin-induced nephrotoxicity in LLC-PK1 cells by blocking the JNKep-53-ecaspase-3 signaling cascade. |
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