| 引用本文:刘若男1，刘文静1，邱建阁2，罗穗豫3.牛蒡子苷元对子宫内膜癌HEC-1B细胞的增殖抑制作用及机制[J].世界中医药,2018,(08):. |
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| 牛蒡子苷元对子宫内膜癌HEC-1B细胞的增殖抑制作用及机制 |
| Proliferation Inhibition Effect of Arctigenin on Endometrial Carcinoma HEC-1B Cell and Its Mechanism |
| 投稿时间：2018-04-16 |
| DOI：10.3969/j.issn.1673-7202.2018.08.046 |
| 中文关键词: 子宫内膜癌 牛蒡子苷元 HEC-1B细胞 增殖 凋亡 NF-κB通路 |
| English Keywords:Endometrial carcinoma Arctigenin HEC-1B cells Proliferation Apoptosis NF-κB pathway |
| 基金项目:河南省科技厅2013年科研项目（201303165） |
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| 中文摘要: |
| 目的：探讨牛蒡子苷元（ARG）对子宫内膜癌HEC-1B细胞的增殖抑制作用及机制。方法：HEC-1B细胞分为观察组和对照组，观察组用终浓度为10、20、30、40、60、100 μmol/L的ARG干预，对照组用0.5%二甲基亚砜（DMSO）干预。48 h后，用四甲基偶氮唑盐微量酶反应比色法（MTT法）检测胞增殖情况；流式细胞仪检测细胞凋亡及细胞周期；蛋白免疫印迹法（WB）检测细胞NF-κB信号通路相关蛋白表达。结果：10、20、30、40、60、100 μmol/L ARG干预48 h，HEC-1B细胞的相对生存率分别为（92.36±0.52）%、（89.59±0.74）%、（78.49±0.68）%、（56.47±0.59）%、（40.12±0.69）%、（37.52±0.58）%，且随着ARG作用浓度的升高，HEC-1B细胞的相对生存率呈逐渐降低趋势，差异有统计学意义（P<0.05），干预48 h HEC-1B细胞50%抑制浓度（IC50）为50 μmol/L。对照组和观察组（50 μmol/L ARG）细胞凋亡率分别为（2.89±0.56）%、（26.58±3.26）%，观察组细胞凋亡率明显高于对照组，差异有统计学意义（P<0.05）。但观察组细胞周期分布与对照组比较，差异无统计学意义（P>0.05）。观察组（50 μmol/L ARG）细胞p65、p-IκB-α蛋白表达量明显低于对照组，差异有统计学意义（P<0.05），2组间IκB-α蛋白表达量差异无统计学意义（P>0.05）。结论：ARG可抑制HEC-1B细胞增殖，促进其凋亡，其作用机制与可能与降低NF-κB通路相关蛋白表达水平，抑制NF-κB通路活化有关。 |
| English Summary: |
| To investigate the proliferation inhibition effect of Arctigenin (ARG) on endometrial carcinoma HEC-1B cell and its mechanism.Methods:HEC-1B cells were divided into test group and control group.The test group was intervened with ARG at the final concentration of 10，20，30，40，60，100 μmol/L，and the control group was intervened with 0.5% dimethyl sulfoxide (DMSO).48 h after incubation，the proliferation was detected by MTT assay.The cell apoptosis and cell cycle were detected by flow cytometry.The expression of NF-κB signaling pathway related proteins were detected by western blotting (WB).Results:The relative survival rates of HEC-1B cell intervened with 10，20，30，40，60 and 100 mol/LARG after 48 h were (92.36±0.52)%，(89.59±0.74)%，(78.49±0.68)%，(56.47±0.59)%，(40.12±0.69)%，(37.52±0.58)% respectively，with the increase of ARG concentration.The relative survival rates of HEC-1B cells decreased gradually (P<0.05).50% inhibitory concentration (ID50) of HEC-1B cells intervened with ARG after 48 h was 50 μmol/L.The apoptosis rates of HEC-1B cells in the control group and the test group (50 μmol/L ARG) were (2.89±0.56)%，(26.58±3.26)% respectively.The apoptosis rate in the test group was significantly higher than that in the control group (P<0.05).However，there was no significant difference in cell cycle distribution between the test group and the control group (P>0.05).The expression levels of p65 and p-IκB-α in the test group (50 μmol/L ARG) were significantly lower than that in the control group (P<0.05).There was no significant difference in the expression levels of IκB-α between the test group and the control group (P>0.05).Conclusion:ARG can inhibit the proliferation and promote apoptosis of HEC-1B cells，and its mechanism may be related to decreasing the expression level of NF-κB related protein and inhibiting the activation of NF-κB pathway. |
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