| 引用本文:田淑霞1,2，李长艳1，张婧娴1，肖霄3，郭澄1，杜江2，韩永龙1,3.东北铁线莲不同提取部位的抗炎镇痛作用研究[J].世界中医药,2018,(08):. |
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| 东北铁线莲不同提取部位的抗炎镇痛作用研究 |
| Study on Anti-inflammatory and Analgesic Effect of Different Fractions Extracted from Clematis manshurica Rupr |
| 投稿时间：2017-11-10 |
| DOI：10.3969/j.issn.1673-7202.2018.08.047 |
| 中文关键词: 东北铁线莲 提取部位 抗炎 镇痛 |
| English Keywords:Clematis manshurica Rupr Extract Anti-inflammation Analgesia |
| 基金项目:上海市科学技术委员会民生科技支撑计划专项（14401972002）；上海市第六人民医院东院人才课题（2014010） |
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| 中文摘要: |
| 目的：研究和比较东北铁线莲不同提取部位的抗炎镇痛活性。方法：东北铁线莲经提取、萃取、浓缩得到石油醚部位、氯仿部位、乙酸乙酯部位和正丁醇部位，实验分为阴性对照组、阳性对照组和实验组，分别利用小鼠醋酸扭体疼痛模型和脂多糖诱导RAW264.7细胞炎性反应模型评估4个提取部位的镇痛活性和抗炎活性。结果：在镇痛实验中，乙酸乙酯部位对小鼠扭体的抑制率最高；抗炎实验结果显示，乙酸乙酯部位抑制NO的效果最为显著。结论：东北铁线莲不同部位的抗炎镇痛效果不同，其中乙酸乙酯部位抗炎镇痛活性最强。 |
| English Summary: |
| To study the anti-inflammatory and analgesic activities of different fractions extracted from Clematis manshurica Rupr.Methods:The parts of petroleum ether，chloroform，ethyl acetate and n-butanol were obtained by abstracting，extracting and concentrating.The experiment was divided into three groups：negative control group，positive control group and experimental group.In the study，mouse acetic acid writhing and lipopolysaccharide (LPS) induced RAW264.7 cell model were used to evaluate analgesic and anti-inflammatory activity of the four fractions.Results:In the experiment of analgesic，the inhibition rate of writhing was the most significant in the ethyl acetate fraction.The results of anti-inflammatory experiment suggested that the ethyl acetate fraction showed significant inhibitory effect on nitric oxide (NO).Conclusion:The anti-inflammatory and analgesic effects of different parts of Clematis mandshurica were different，and ethyl acetate fraction showed the most significant anti-inflammatory and analgesic activity. |
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