| 引用本文:蒋洪亮1，赵修南2，麻浩2，何丽芳3，单俊杰2，王海南1,4.发酵虫草菌粉多糖免疫活性实验研究[J].世界中医药,2018,(08):. |
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| 发酵虫草菌粉多糖免疫活性实验研究 |
| Experimental Study on Immune Activities of Polysaccharides from Fermented Cordycepssinensis Powders |
| 投稿时间：2018-03-31 |
| DOI：10.3969/j.issn.1673-7202.2018.08.050 |
| 中文关键词: 发酵虫草菌粉 多糖 细胞增殖 脾细胞 巨噬细胞 免疫活性 肿瘤坏死因子 γ-干扰素 |
| English Keywords:Fermented Cordycepssinensis powders Polysaccharides Cell proliferation Spleen cells Macrophages Immune activity TNF-α IFN-γ |
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| 中文摘要: |
| 目的:探究发酵虫草菌粉多糖的免疫活性。方法:发酵虫草菌粉通过水提醇沉、复溶、透析、浓缩、冷冻干燥得多糖,命名为CSMP-60,其提取条件:60 ℃水提、提取时间4 h、料液比1:15、提取次数2次。以小鼠免疫细胞为材料,检测CSMP-60的免疫活性。结果:CSMP-60能促进脾细胞的增殖(P<0.05),明显促进小鼠腹腔巨噬细胞分泌TNF-α(P<0.05),有促进脾淋巴细胞分泌IFN-γ的趋势。结论:CSMP-60可能是发酵虫草菌粉产生免疫活性的效应物质之一。 |
| English Summary: |
| To investigate the immunological activity of polysaccharides from fermented Cordycepssinensis powders.Methods:Polysaccharides from fermented Cordycepssinensis powders were obtained through water extraction and alcohol precipitation,reconstitution,dialysis,concentration and freeze-drying,named after CSMP-60.Its extraction conditions：60 ℃ water extraction,extraction time 4h,solid-liquid ratio 1:15,the number of extractions 2 times.The immune activity of CSMP-60 was measured using mouse immune cells.Results:CSMP-60 could promote the proliferation of spleen cells (P<0.05),and significantly promote the secretion of TNF-α from mouse peritoneal macrophages (P<0.05).It has a tendency to promote the secretion of IFN-γ by spleen cells.Conclusion:CSMP-60 may be one of the immunocompetence substances of fermented Cordycepssinensis powders. |
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