| To observe the effects of Baohe Pills on blood lipid and intestinal microbiota in SD rats with a high-fat diet to provide reference for clinical application and experimental research.Methods:A total of 60 rats were randomly divided into 4 groups:normal group,model group,high-fat diet with Baohe pills group (HB group) and the normal diet with Baohe pills group (NB group).The serum lipid level was detected by Elisa method,and the intestinal microbiota of rats was analyzed based on 16S rDNA gene sequencing technique.Results:the contents of serum cholesterol (TC),triglyceride (TG) and low-density lipoprotein (LDL-C) in the rats of HB group were decreased more significantly than those of the model group,but less than those of the normal group and the NB group.There was no statistically significant difference between the normal group and the NB group.The results of intestinal microbiota analysis showed no significant difference in number of fecal microbial flora between the NB group and the normal group.Compared with the model group,the numbers of Bifidobacteriales,Clostridiales,Desulfovibrionales,and RF9 bacteria of HB group decreased more than that of the normal group and the NB group.In the meantime,compared to the model group,the HB group experienced a significantly reduction in the number of Coriobacteriales,Bacteroidales,Bacillales,Lactobacillales,Erysipelotrichales,Burkholderiales,Aeromonadales,Verrucomicrobiales,etc.,but the number was still higher than that of the normal group and the NB group.Conclusion:Baohe pills may reduce TC,TG and LDL-C by altering the diversity of intestinal microbiota. |
