To explore proteomics characteristics of chronic heart failure (CHF) with qi deficiency and blood stasis syndrome and qi and yin deficiency syndrome and possible biological pathways of the mechanism.Methods:This study was designed to divide the participants into CHF with qi deficiency and blood stasis syndrome group,qi and yin deficiency syndrome group,healthy control group.Quantitative iTRAQ marker as a new quantitative proteomics technology combined with tandem mass spectrometry was used to analyze the differential protein expression of CHF with qi deficiency and blood stasis syndrome group and qi and yin deficiency syndrome,which may provide experimental evidence for the disease and biological basis in protein level,and reveal the content of syndrome in a certain level.Results:Compared with healthy control group,a total of 16 kinds of protein had differential expression in qi deficiency and blood stasis group.The expressions of apolipoprotein E,galectin-3-binding protein and other 9 kinds of proteins were up-regulated.The expressions of vitamin D-binding protein (DBP),trypsin inhibitor and other 3 proteins were down-regulated.Compared with health control group,there were 15 kinds of proteins which had differential expressions in qi and yin deficiency group,and complement 9,inter-alpha-trypsin inhibitor family heavy chain-related protein (IHRP) and other 8 proteins expressions were up-regulated.The expressions of pre-serum amyloid P component,DBP and other 3 proteins were down-regulated.Conclusion:Proteomic detection results can reflect the biological basis of CHF qi deficiency and blood stasis syndrome,qi and yin deficiency syndrome factor,which can objectively evaluate characteristics of this syndrome and provide new view of combined research of disease and syndrome.