| 引用本文:李君玲1，2，马雪玲2，李玉波3，夏恺2，王田2，薛晓兴2，赵慧辉2，王伟2.基于脑功能核磁的抑郁症肝郁脾虚证大鼠中枢分子生物学基础研究[J].世界中医药,2018,(09):. |
|
| 基于脑功能核磁的抑郁症肝郁脾虚证大鼠中枢分子生物学基础研究 |
| Basic Study on Molecular Biology of Depression with Stagnation of Liver Qi and Spleen Deficiency in Rats Based on Functional Magnetic Resonance Imaging |
| 投稿时间：2018-04-20 |
| DOI：10.3969/j.issn.1673-7202.2018.09.008 |
| 中文关键词: 肝郁脾虚 抑郁症 生物学基础 功能核磁 |
| English Keywords:Depression with stagnation of liver qi and spleen deficiency syndrome Depression Biological basis Functional magnetic resonance imaging |
| 基金项目:国家自然科学基金青年项目（81703863）；国家重点基础研究发展计划（973计划）项目(2011CB505106) |
|
| 摘要点击次数: 731 |
| 全文下载次数: 1049 |
| 中文摘要: |
| 目的：研究抑郁症肝郁脾虚证大鼠中枢分子生物学基础。方法：慢性不可预知温和应激方法对大鼠进行造模，磁共振功能成像技术扫描大鼠全脑信息，利用SPM8软件计算出抑郁症肝郁脾虚证大鼠与正常组比较在低频震荡（Amplitude of the Low-frequency Fluctuations，ALFF)和局部一致性（Regional Homogeneity，ReHo）中存在异常的脑区；高效液相色谱法对所探索的密切相关脑区进行单胺类神经递质检测；四逆散方剂反证确定其中枢分子生物学基础。结果：模型大鼠下丘脑Reho与ALFF均减弱，下丘脑DA、5-HT、5-HIAA和NE含量明显降低，差异有统计学意义（P<0.05），四逆散组较模型组比较下丘脑5-HT含量显著升高（P<0.05），NE、DA、5-HIAA存在升高趋势，差异有统计学意义（P>0.05）。结论：下丘脑5-HT、5-HIAA、NE、DA是肝郁脾虚型抑郁症的分子生物学基础之一。 |
| English Summary: |
| To explore the central molecular biological basis of depression with stagnation of liver qi and spleen deficiency syndrome in rats.Methods:The chronic unpredictable mild stress (CUMS) method was used to establish the model.Rat's brain information was scanned with functional magnetic resonance imaging (fMRI).The amplitude of the low-frequency fluctuations (ALFF) and the abnormal brain region in regional homogeneity (Reho) of rats in normal group and depression with stagnation of liver qi and spleen deficiency syndrome group were calculated and analyzed by SPM8.High performance liquid chromatography（HPLC）was used to detect the monoamine neurotransmitter change of the close related brain region.Sini Powder was used to confirm the biological basis from the perspective of counterevidence.Results:Both ALFF and Reho in the hypothalamus were decreased.The contents of dopamine (DA),serotonin (5-HT),and 5 hydroxyindole acetic acid (5-HIAA) and norepinephrine (NE) were reduced significantly (P<0.05).The content of 5-HT of Sini Powder group increased more significantly than that of normal group (P<0.05).There was improvement tendency in the DA,5-HIAA,and NE (P>0.05).Conclusion:The 5-HT,5-HIAA,NE,DA in the hypothalamus were parts of the central biological basis of depression with stagnation of liver qi and spleen deficiency syndrome. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
