To study the effects of Jianpi Ruangan Formula on the expression of α-smooth muscle actin (α-SMA) and m-RNA in rats with hepatic fibrosis induced by carbon tetrachloride (CCl4),to preliminarily discuss the mechanism of Jianpi Ruangan Formula in preventing and treating liver fibrosis,and to provide experimental basis for clinic treatment of hepatic fibrosis in traditional Chinese medicine.Methods:A total of 72 Wistar male rats were selected and randomly divided into normal group,model group,Fuzheng Huayu Capsule group,Jianpi Ruangan Formula high,middle,low dose group.Rats in model group was intraperitoneally injected with the mixed solution of 30% CCl4 and 70% olive oil at 0.2 mL/100 g while the normal group was intraperitoneally injected with the same dose of saline.Each group injected twice a week for 4 weeks,and the animal models of rat liver fibrosis was replicated after 4 weeks.The expression of α-SMA in rats with hepatic fibrosis was detected by ABC immunohistochemical method and expression of the m-RNA was determined by Real-time PCR method.The HE staining and Mallory staining were applied to observe the pathological changes of hepatic fibrosis in rats.Results:The expression of α-SMA protein in model group increased (P<0.05).Compared with the Jianpi Ruangan formula high,middle,low doze groups,the difference was statistically significant (P<0.05).Among them,the positive expression of α-SMA protein in the high,middle and low dose groups gradually increased,showing some signs of drug dependence.Compared with the Jianpi Ruanfang formula high,middle,low doze groups,the expression of α-SMA mRNA in model group significantly increased (P<0.05),and there was significant difference (P<0.05).The expression of α-SMA mRNA in the high,middle and low dose groups gradually increased,showing some signs of drug dependence.Conclusion:The mechanism of the Jianpi Ruangan Formula against hepatic fibrosis may be related to the inhibition of the expression of α-SMA and m-RNA in rat liver.