To study the effect of decocting time on the active components of Zhizi Baipi decoction, and provide the scientific basis for quality control and clinical application of Zhizi Baipi decoction. Methods:HPLC-DAD was employed to determine the content of geniposide-genipin1gentiobioside-magnoflorine-liquiriti-n (λ=238 nm), glycyrrhizic acid-berberine hydrochloride-palmatinechloride (λ=265 nm), isliquiritin (λ=360 nm), crocinⅠ-crocinⅡ (λ=440 nm) in Zhizi Baipi decoction at various decocting time. Results:When the decocting time changes, each ingredient has different variation trend; Fructus gardeniae, Phellodendron amurense, Glycyrrhica uralensis and the total active components have significant difference; Fructus gardeniae, Phellodendron amurense, Glycyrrhica uralensis and the total active components have significant rise during 10～20 min, then decrease in certain extent and increase later. The final content approach the content at 20 min. Conclusion:Different decocting time changed the active components of Zhizi Baipi decoction, thus changed its efficacy; when the decocting time for 20 min, each active component has higher content and it avoids the destruction of crocin by long time heat, which proved the rationality of traditional decocting time.