世界中医药

目的：采用高效液相（HPLC）比较研究葛根芩连汤配方颗粒剂和传统汤剂的指纹图谱差异。方法：首先选择3家不同医院A、B、C的葛根芩连汤配方颗粒剂和传统汤剂，配制对照品溶液和供试品溶液后，以优化的色谱条件进行HPLC指纹图谱研究。结果：HPLC中间精密度试验、重复性试验和稳定性试验均显示良好。葛根芩连汤颗粒剂HPLC指纹图谱发现前5 min K1、K2和K3的谱图差异较大，5 min后3家不同医院葛根芩连汤颗粒剂HPLC指纹图谱有10个相似的特征峰，其中K3特征峰的成分含量与K1和K2存在较大差异，尤其是1号峰和4号峰。K1和T1、K2和T2、K3和T3颗粒剂和传统汤剂的比较中，颗粒剂特征峰的含量较传统汤剂高。结论：葛根芩连汤配方不同医院颗粒剂和传统汤剂的HPLC指纹图谱存在一定差异。

English Summary:

To compare the fingerprints of Gegen Qinlian Formula granules and its traditional decoction by high performance liquid phase (HPLC).Methods:Gegen Qinlian Decoction Formula granules and traditional decoctions from 3 different hospitals A,B,C,were selected.After preparing the control solution and test solution,the HPLC fingerprint analysis was carried out under the optimized chromatographic conditions.Results:The results of HPLC intermediate precision test,repeatability test and stability test were all good.Gegen Qinlian Decoction granules HPLC fingerprint showed that the spectra of K1,K2 and K3 differed greatly during the first 5 min.Then after the 5 min,HPLC fingerprint of Gegen Qinlian Decoction Formula granules from 3 different hospitals had 10 similar characteristic peaks,the content of K3 characteristic peak was quite different from K1 and K2,especially No.1 and No.4 peaks.In comparison of K1 and T1,K2 and T2,K3 and T3 of granules and traditional decoctions,the content of the characteristic peak of granules was higher than that of traditional decoctions.Conclusion:There are certain differences in the HPLC fingerprints of Gegen Qinlian Decoction granules and traditional decoction from different hospitals.

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