| 引用本文:郝彬，赵红果.牛蒡子苷元对体外C6胶质瘤细胞的抑制效果和凋亡机制[J].世界中医药,2018,(09):. |
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| 牛蒡子苷元对体外C6胶质瘤细胞的抑制效果和凋亡机制 |
| Research on Arctigenin in Proliferation and Apoptosis of C6 Glioma Cells |
| 投稿时间：2017-04-07 |
| DOI：10.3969/j.issn.1673-7202.2018.09.053 |
| 中文关键词: 牛蒡子苷元 C6胶质瘤细胞 细胞增殖 细胞凋亡 |
| English Keywords:Arctigenin C6 glioma cell Cell proliferation Cell apoptosis |
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| 中文摘要: |
| 目的：探讨牛蒡子苷元对体外C6胶质瘤细胞的抑制效果和凋亡机制。方法：将牛蒡子苷元作用于C6胶质瘤细胞，采用CCK-8法测定各组细胞的生长抑制率；流式细胞术检测细胞凋亡情况；酶联免疫吸附试验检测TNF-α、IL-2的水平；Western blot检测Bax、Bcl-2、NF-кB蛋白表达水平。结果：与空白组比较，牛蒡子苷元组（2.5～40 μmol/L）显著抑制C6胶质瘤细胞生长，诱导肿瘤细胞凋亡，其诱导效应具有浓度依赖性；牛蒡子苷元处理后，细胞中TNF-α、IL-2水平增加；牛蒡子苷元可以显著上调Bax蛋白表达，下调Bcl-2、NF-кb蛋白表达。结论：牛蒡子苷元可以促进C6胶质瘤细胞凋亡，可能是通过增强TNF-α、IL-2水平及上调Bax蛋白、下调Bcl-2、NF-кB蛋白水平而诱导肿瘤细胞凋亡。 |
| English Summary: |
| To investigate the inhibitory effect and apoptosis mechanism of arctigenin on C6 glioma cells. Methods:Arctigenin acted on C6 glioma cells. CCK-8 was used to detect the growth inhibition rate of each group; flow cytometry was used to detect apoptosis rate of different group; the levels of TNF-α and IL-2 were detected by ELISA; the expressions of Bax, Bcl-2 and NF-кb proteins were analyzed by Western blot. Results:Compared with the blank group, arctigenin (2.5～40 μmol/L) significantly inhibited the growth of C6 glioma cells and induced apoptosis of tumor cells in concentration-dependent; after treatment with arctigenin, the levels of TNF-α and IL-2 in the cells were increased; arctigenin could up-regulate the expression of Bax protein and down-regulate the expression of Bcl-2 and NF-кb Proteins. Conclusion:Arctigenin can promote the apoptosis of C6 glioma cells, which maybe through enhance the levels of TNF-α, IL-2 and up-regulated Bax protein, down-regulate Bcl-2 and NF-кb proteins levels. |
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