| 引用本文:聂宇1，周纳新1，刘健1，万峰1，傅德皓2.丹参酮ⅡA对大鼠急性脊髓损伤SOCS3/STAT3信号通路的影响[J].世界中医药,2018,(10):. |
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| 丹参酮ⅡA对大鼠急性脊髓损伤SOCS3/STAT3信号通路的影响 |
| Effects of Tanshinone ⅡA on SOCS3/STAT3 Signaling Pathway in Rats with Acute Spinal Cord Injury |
| 投稿时间：2018-09-03 |
| DOI：10.3969/j.issn.1673-7202.2018.10.046 |
| 中文关键词: 丹参酮ⅡA 急性脊髓损伤 细胞因子信号抑制因子3 信号转导与转录激活子3 |
| English Keywords:Tanshinone ⅡA Acute spinal cord injury Suppressors of cytokine signaling-3 Signal transducer and activator of transcription-3 |
| 基金项目:宜昌市科技局项目（A15301-04） |
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| 中文摘要: |
| 目的：通过观察丹参酮ⅡA对大鼠急性脊髓损伤后细胞因子信号抑制因子3(Suppressors of Cyto-kine Signaling-3，SOCS3)/信号转导与转录激活子3（Signal Transducer and Activator of Transcription-3,STAT3）信号通路表达的影响，探讨丹参酮ⅡA对大鼠急性脊髓损伤炎性反应的影响。方法：60只健康SD大鼠随机分为脊髓损伤组（对照组）、脊髓损伤/丹参酮IIA治疗组（观察组）2组，每组30只。制作脊髓损伤动物模型。各组分别在脊髓损伤后8 h、1 d、3 d、7 d、14 d取材。分析大鼠脊髓损伤后运动诱发电位（MEP）的潜伏期及波幅；RT-PCR半定量分析脊髓组织中SOCS3 mRNA、STAT3 mRNA的表达；TUNEL法行神经细胞凋亡检测。结果：与对照组比较，观察组MEP潜伏期差异无统计学意义（P>0.05），MEP波幅则明显增高（P<0.05）；SOCS3 mRNA表达明显增高、STAT3mRNA表达明显下降；神经细胞凋亡有所减少(P<0.05)。结论：丹参酮IIA可提高SOCS3表达，抑制STAT3表达，抑制神经细胞的凋亡，减轻神经功能缺失，对急性脊髓损伤大鼠有一定的保护作用。 |
| English Summary: |
| To observe the effects of Tanshinone ⅡA on the expression of suppressors of cytokine signaling-3,(SOCS3)/signal transducer and activator of transcription-3 (STAT3) in rats with acute spinal cord injury.Methods:A total of 60 healthy adult Sprague-Dawley rats were randomly divided into two groups:control group and Tanshinone ⅡA group.The acute spinal cord injury model was built in rats.At 8 h,1 d,3 d,7 d,and 14 d after spinal cord injury,we analyzed latency and amplitude of MEP (Motion Evoked Potential),and reverse transcription polymerase chain reaction (RT-PCR) was used to detect the mRNA expressions of SOCS3 and STAT3 in spinal cord tissues.Apoptosis neurons were labeled with TUNEL dyeing.Results:There were no changes in the latencies of MEPs in both groups,and the mean amplitudes of MEPs were higher in Tanshinone ⅡA group than those in control group.The expressions of SOCS3 mRNA and STAT3 mRNA were increased in Tanshinone ⅡA group compared with control group.The number of TUNEL staining positive cells in the Tanshinone ⅡA group was less than that in control group (P<0.05).Conclusion:Tanshinone ⅡA may enhance the expression of SOCS3 and inhibit the expression of STAT3,inhibit neurocyte apoptosis and palliate loss of neural function.It has certain protective effects on rats with acute spinal cord injury. |
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