| 引用本文:纪雯婷1，尹湘君1，刘姝伶1，张海霞1，王雪茜2，魏玮2.栀子苷干预LPS诱导RAW264.7巨噬细胞炎性反应递质的研究[J].世界中医药,2018,(10):. |
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| 栀子苷干预LPS诱导RAW264.7巨噬细胞炎性反应递质的研究 |
| Study on Geniposide Intervening LPS-induced Inflammatory Mediators in RAW264.7 Macrophages |
| 投稿时间：2017-12-17 |
| DOI：10.3969/j.issn.1673-7202.2018.10.054 |
| 中文关键词: 栀子苷标准品 栀子水提液 栀子敲除液 LPS 巨噬细胞 IL-6 TNF-α NO |
| English Keywords:Geniposide standard Gardenia aqueous extract Geniposide-knockout liquid LPS Macrophages IL-6 TNF-α NO |
| 基金项目:国家自然科学基金项目(81373886)——利用单克隆抗体特异性敲除技术解析栀子苷与栀子药效关联性的研究 |
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| 中文摘要: |
| 目的:通过比较栀子苷水提液、栀子苷你标准品与栀子苷敲除液对LPS刺激巨噬细胞产生炎性反应的干预,探究栀子主要成分栀子苷的抗炎作用。方法:分别检测栀子苷水提液、栀子苷标准品、50%栀子苷敲除液对巨噬细胞的毒性;设立空白对照组、LPS模型组、栀子苷水提液组、栀子苷标准品组、50%栀子苷敲除液组、50%栀子苷回填组,检测各组对LPS刺激巨噬细胞后释放NO以及分泌IL-6和TNF-α的干预作用比较。结果:LPS刺激巨噬细胞后NO释放增多(P<10-4),而栀子苷水提液、栀子苷标准品、栀子苷敲除液均能降低LPS刺激巨噬细胞后NO的释放,其中栀子水提液、50%栀子苷敲除液以及50%栀子苷回填液的作用最强,且其作用差异无统计学意义(P<10-2)。而栀子苷标准品的作用相对较小(P<0.05)。LPS刺激巨噬细胞后,巨噬细胞分泌TNF-α与IL-6较空白对照组增加(P<10-4),栀子水提液干、栀子苷标准品、50%栀子苷敲除液以及含50%栀子苷的栀子水提液干预后,能降低上述细胞因子的分泌(P<0.05)。结论:栀子中栀子苷与其他成分均具有一定的抗炎作用,各成分之间的抗炎作用各有侧重。 |
| English Summary: |
| To explore the anti-inflammatory effects of geniposide,a main component of Fructus Gardeniae,by comparing the intervention in aqueous extract of gardenoside,the standard product of geniposide and geniposide-knockout liquid on LPS-stimulated macrophage.Methods:Geniposide aqueous extract,geniposide standard product and 50%-geniposide-knockout liquid were used to detect macrophage cytotoxicity.The control group,LPS model group,and aqueous extract of geniposide liquid group,geniposide standard product group,50%-geniposide-knockout liquid group and 50%-geniposide-backfill liquid group were set up separately.The levels of NO,IL-6 and TNF-α in each group were detected in LPS-stimulated macrophages.Results:NO release was increased in LPS-stimulated macrophages (P<10-4),and aqueous extract geniposide,geniposide standard product,and geniposide-knockout liquid reduced the release of NO.Among them,the effects of geniposide aqueous extract,50%-geniposide-knockout liquid and 50%-geniposide-backfill liquid were the strongest (P<10-2),and there was no significant difference.The effects of geniposide standard product was relatively small (P<0.05).After stimulated by LPS,macrophages secreted more TNF-α and IL-6 than the control group (P<10-4),while geniposide aqueous extract,geniposide standard product,50%-geniposide-knockout liquid and 50%-geniposide aqueous extract could reduce the secretion of cytokines (P<0.05).Conclusion:Geniposide and other components of gardenia have a certain anti-inflammatory effect,and the anti-inflammatory effects of the various components have different emphases. |
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