| 引用本文:陈菲，冯英楠，张鹏，庄伟，林晓兰.医疗机构制剂活血益气方抗瘤丸基于裸鼠荷瘤模型的工艺筛选[J].世界中医药,2018,(10):. |
|
| 医疗机构制剂活血益气方抗瘤丸基于裸鼠荷瘤模型的工艺筛选 |
| Technology Screening of Qi Supplement and Blood Activation Prescription Kangliu Pills Prepared by Medical Institutes Based on Nude Mice Tumor-Bearing Model |
| 投稿时间：2017-01-03 |
| DOI：10.3969/j.issn.1673-7202.2018.10.057 |
| 中文关键词: 抗瘤丸 神经胶质瘤 裸鼠 抑瘤 工艺 |
| English Keywords:Kang Liu Wan Neuroglioma Nude mice Anti-tumor Preparation technology |
| 基金项目:北京市科委十病十药（Z141100002214016）；北京市教委2017年度首都中医药研究专项（1ZY13）；2016年北京市中医药科技发展资金项目（JJ2016-10） |
|
| 摘要点击次数: 808 |
| 全文下载次数: 635 |
| 中文摘要: |
| 目的：筛选医疗机构制剂活血益气方抗瘤丸（简称KLW）的制备工艺。方法：将足够量的大鼠神经胶质瘤C6细胞注射至裸鼠右侧腋窝，建立裸鼠荷瘤模型，按瘤块体积随机分组，分别灌胃给予等量的不同工艺KLW1～4，以替莫唑胺为阳性对照，以溶媒（去离子水）为模型对照，测量裸鼠体重、瘤体积，并观察给药前后小鼠症状；连续给药15 d后，处死，称量裸鼠瘤重量。结果：KLW-1中、高剂量组裸鼠瘤块重量，KLW-1高剂量组瘤体积，均低于模型对照组，差异有统计学意义（P<0.05），阳性对照组瘤块重量及瘤体积均低于模型对照组，差异有统计学意义（P<0.01）。其他处方组裸鼠瘤块重量、体积与模型对照组比较，差异无统计学意义（P>0.05）。各待测组（KLW-1~4高、中、低剂量组）裸鼠体重与模型对照组比较，差异均无统计学意义（均P>0.05），阳性对照组给药后裸鼠体重明显低于模型对照组，差异有统计学意义（P<0.01）。各组有少量裸鼠死亡。结论：抗瘤丸在荷瘤裸鼠模型中具有一定抑瘤效果，与待测工艺比较，原制剂工艺抑瘤效果较好，成本低廉，适用于大规模现代化生产。 |
| English Summary: |
| To screen the preparation technology of qi reinforcement and blood circulation promotion prescription-Kang Liu Wan (KLW) for medical institutes.Methods:A sufficient amount of C6 mice neuroglioma cell were injected into nude mice right armpit to establish a Nude Mice Tumor-Bearing model.Experimental mice were randomly divided into groups based on model tumor size and intragastrically administered with equal amounts of KLW1-4.Temozolomide was set as a positive control and vehicle (deionized water) as a model control.The nude mice weight and tumor size were measured,and the symptoms of mice was observed during the whole experiment.After 15 day's experiment,the tumor was weighed.Results:The tumor weight of KLW-1 middle and high dose group were lower than those in the model control group,and the difference was statistically significant (P<0.05).The tumor weight and tumor size in the positive group were lower than those in the model control group,and the difference was statistically significant (P<0.01).Compared with control group,all other prescription groups had no significant difference in the weight and size of tumor,and the difference was not statistically significant (P>0.05).And all preparation technology test groups had no statistically significant difference in weight (P>0.05) when compared with control group.The positive control group was significantly decreased when compared with control group after treatment,and the difference was statistically significant (P<0.01).A small number of nude mice died in each group.Conclusion:KLW has a specific anti-tumor effect in Nude Mice Tumor-Bearing model.Compared with preparation test technology,traditional prescription process technology has advantage in anti-tumor effect,prescription cost and mass-production technologies adaptability |
| 查看全文 查看/发表评论 下载PDF阅读器 |
