To study the composition of the drugs in Gegen Qin Lian Decoction by HPLC (high performance liquid) fingerprint.Methods:HPLC-DAD was used in the fingerprint research method (high performance liquid chromatography with diode array detector),HPLC fingerprint chromatography test conditions: sample volume of 20 L,Ultimate AQ C18 column with diameter of 5 m column,the column temperature was 30 degrees,the mobile phase is A 0.02 mol/L +0.3% three +1% ammonium acetate ethylamine acetic acid mixture,the final pH was 4.3;the mobile phase is B acetonitrile,0.7 mL/min uniform velocity.The detection wavelength by constantly adjusting the final pre experiment,puerarin,daidzin,daidzein,genistein,ammonium glycyrrhizinate and glycyrrhetinic acid test wavelength was 250 nm; baicalin,wogonoside,baicalein,wogonin and liquiritin detection wavelength was 280 nm; the remaining standard detection wavelength was 346 nm;and the HPLC precision test,repeatability test,stability test,specificity test,recovery test,linear regression test methodology for rigorous investigation,through the comparison of mixed standard solution of fingerprint,gegenqinliantang single fingerprint and gegenqinliantang fingerprint analysis of Radix Puerariae Qinlian Decoction of 14 ingredients.Results:1)The average relative peak area RSD of precision test was 0.38%,the average relative peak area RSD of repeatability test was 1.14%,the average relative peak area RSD of stability test was 1.03%,and the specificity test result was good.2)The linear regression of 14 drug components is good,and the range of average recovery in the sample recovery test is 96.45%～104.69%,and the range of RSD is 0.65%～2.11%.3)According to different detection wavelengths 250 nm,280 nm and 346 nm,the fingerprint of mixed reference products was divided into 3 diagrams,which was convenient for subsequent analysis,and 14 peaks of reference compounds were also labeled.4)At the detection wavelength of 250 nm,there were 11 characteristic peaks in the fingerprint of radix puerariae,while 2 characteristic peaks in Glycyrrhiza uralensis Fisch.There were 6 characteristic peaks in Scutellaria baicalensis Georgi at the detection wavelength 280,while there were 4 characteristic peaks in Rhizoma Coptidis at the detection wavelength of 346 nm.5)There are 12 characteristic peaks in Gegenqinlian Decoction fingerprint displayed in the wavelength of 250 nm,characteristics of the 1~11 drug ingredients mainly puerarin and 12 among them,mainly licorice ingredient characteristics;280 nm wavelength of 7 peaks,mainly reflected the drug composition characteristics of liquorice and Radix Scutellariae; wavelength 346 nm there are 4 characteristic peaks,mainly reflected the drug composition characteristics of Coptis chinensis.Conclusion:The source of drug composition of Gegen Qin Lian Decoction HPLC fingerprint is analyzed integrally,providing experimental basis for the material basis and pharmacological correlation research of Gegen Qin Lian decoction.