| 引用本文:周建衡1，杨弘2，林久茂2，洪振丰1.前列宁胶囊调控MMP-2影响细胞外基质对良性前列腺增生治疗的影响[J].世界中医药,2018,(11):. |
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| 前列宁胶囊调控MMP-2影响细胞外基质对良性前列腺增生治疗的影响 |
| Effects of Qianliening Capsules in Regulating Extracellular Matrix via MMP-2 on Treating Benign Prostatic Hyperplasia |
| 投稿时间：2018-03-05 |
| DOI：10.3969/j.issn.1673-7202.2018.11.041 |
| 中文关键词: 前列腺增生 前列宁胶囊 ECM MMP-2 FN Collagen IV LN Q-PCR Western-Blot |
| English Keywords:Benign prostatic hyperplasia Qianliening capsule Extracellular matrix MMP-2 FN Collagen IV LN Q-PCR Western-Blot |
| 基金项目:福建省自然基金项目(2015J01333);国家自然科学基金项目(81173433) |
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| 中文摘要: |
| 目的:观察前列宁胶囊(QC)对基质金属蛋白酶-2(MMP-2)介导的细胞外基质(ECM)的影响,探讨QC对BPH的治疗机制。方法:SD大鼠随机分为空白组,BPH模型组,QC低、中、高观察组,建立BPH模型,QC干预,ELISA法检测大鼠血清MMP-2、FN、Collagen IV、LN;BPH-1细胞培养,分别加入MMP-2和未加MMP-2刺激因子,QC干预,MTT法观察细胞活性,Q-PCR及Western-Blot分别检测MMP-2、FN、Collagen IV、LN基因及蛋白表达(未加MMP-2),加入MMP-2组检测FN、Collagen IV、LN。结果:QC各剂量组大鼠血清中的FN、LN、Collagen IV含量降低,MMP-2升高(P<0.05 or P<0.01);BPH-1细胞培养QC干预,加入及未加入MMP-2刺激因子细胞活力均有下降,以48 h后最明显;加入MMP-2刺激因子FN、Collagen IV、LN基因及蛋白表达明显低于未加MMP-2刺激因子,差异有统计学意义。结论:QC对BPH具有治疗作用,QC调控MMP-2介导细胞外基质FN、Collagen IV、LN基因和蛋白表达可能是其治疗BPH机制之一。 |
| English Summary: |
| To observe the effects of Qianliening Capsules (QC) on the extracellular matrix (ECM) via MMP-2 in treating benign prostatic hyperplasia (BPH)，and to explore the therapeutic mechanism of QC on BPH.Methods:Sprague-Dawley rats were randomly divided into blank group，BPH model group，QC low，middle，and high treatment groups.BPH rat model was established and intervened by QC.The MMP-2，LN，FN，Collagen IV in serum were examined by ELISA.BPH-1 cells without MMP-2 stimulators and BPH-1 cells added with MMP-2 stimulators were cultured respectively and treated by QC.The cell morphology and viability were observed by light microscope and MTT.The mRNA and protein expression of MMP-2，LN，Collagen IV and FN were detected by Q-PCR and Western blot assays (no MMP-2)，LN，Collagen IV and FN were detected by Q-PCR and Western blot assays (MMP-2 was added).Results:The levels of Collagen IV，FN，LN in serum of BPH rats serum treated with QC groups were reduced significantly and MMP-2 was significantly increased (P﹤0.05 or P﹤0.01).The mRNA and protein expression of LN，FN，Collagen IV in BPH-1 cells QC-treated groups were decreased significantly，and the result was more obvious in the MMP-2 added groups，but the MMP-2 was enhanced in BPH-1 cells QC-treated groups，with significant difference.Conclusion:QC has significant therapeutic effects on BPH.QC can inhibit the expression of FN，Collagen IV and LN，and enhance MMP-2，and MMP-2 can regulate FN，Collagen IV and LN，QC regulate the expression of ECM via MMP-2 is one of the mechanisms by which QC treats BPH. |
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