To discuss the effects of serum acetyl choline and amyloid-beta protein by using acupuncture Baihui (GV20) and Yongquan (KI1) acupoint to treat patients with Alzheimer disease (AD).Methods:A total of 120 patients with AD in Affiliated Hospital of Liaoning University of Traditional Chinese Medicine from January 2015 to June 2016 were selected and divided into observation group and control group with 55 cases in each group according to the random indicator method.The control group received placebo.Based on the control group，the observation group received acupuncture Baihui (GV20) and Yongquan (KI1) acupoint treatment.Changes in scores of Mini-Mental State Examination (MMSE) and Revised Hasegawa dementia scale (HDS-R)，plasma levels of acetyl choline (Ach)，and amyloid-beta protein (Aβ1-40、Aβ1-42) of two groups were observed before and after treatment.Results:Before treatment，various observation indexes of two groups was basically the same，without statistical significance (P>0.05).After treatment，scores of MMSE and HDS of observation group were significantly higher than those of control group，with statistical significance (P<0.05).The plasma levels of Aβ1-40 and Aβ1-42 of observation group were significantly higher than those of control group，with statistical significance (P<0.05).Plasma levels of Ach in observation group were significantly lower than those of control group，with statistical significance (P<0.05).As Spearman correlation analysis showed，scores of MMSE were negatively correlated with plasma levels of Aβ1-40 and Aβ1-42 (P<0.05)，and scores of MMSE were positively correlated with plasma levels of Ach (P<0.05).The scores of HDS were negatively correlated with plasma levels of Aβ1-40 and Aβ1-42 (P<0.05)，and scores of HDS were positively correlated with plasma levels of Ach (P<0.05).Conclusion:Acupuncture Baihui (GV20) and Yongquan (KI1) acupoint can be effective in the treatment of AD，whose molecular biology mechanism may be improvement of the plasma levels of Ach，decrease of the plasma levels of Aβ1-40 and Aβ1-42.