| 引用本文:刘欣，赵京霞，王燕，李萍.紫草素对银屑病T淋巴细胞增殖、活化的影响[J].世界中医药,2018,(11):. |
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| 紫草素对银屑病T淋巴细胞增殖、活化的影响 |
| Effects of Shikonin on Proliferation and Activation of T Lymphocytes in Psoriasis |
| 投稿时间：2018-05-24 |
| DOI：10.3969/j.issn.1673-7202.2018.11.049 |
| 中文关键词: 紫草素 T淋巴细胞活化 信号转导通路 银屑病 凉血解毒 |
| English Keywords:Shikonin T lymphocyte activation Signal transduction pathway Psoriasis Treatment principle of cooling blood and detoxicification |
| 基金项目:国家自然科学基金项目（81573974,81673974）；北京市自然科学基金项目（7172097） |
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| 中文摘要: |
| 目的：研究具有凉血、解毒作用的中药单体紫草素对银屑病病理状态下异常活化的T淋巴细胞功能及相关信号转导通路的影响。方法：选取Jurkat E6-1 T淋巴细胞，以佛波醇酯和离子霉素活化Jurkat E6-1 T淋巴细胞，同时将0.5~2 μg/mL浓度的紫草素作用于细胞，采用CCK-8法检测T淋巴细胞增殖能力；流式细胞术检测细胞膜CD69的表达及细胞内游离钙离子浓度（［Ca2+］i）的变化；用酶联免疫吸附试验（ELISA）检测T淋巴细胞释放白细胞介素-2(IL-2)、干扰素-γ(IFN-γ)、肿瘤坏死因子-α(TNF-α)的水平；用Realtime-PCR和Western blotting法分别观察核转录因子mRNA及蛋白的表达影响。结果：不同浓度的紫草素均可明显抑制细胞增殖、CD69表达及Th1类细胞因子分泌；均可有效降低［Ca2+］i和蛋白激酶C（PKC）磷酸化蛋白的水平；显著降低核转录因子NF-AT mRNA的表达，下调c-Jun的mRNA及蛋白表达并可抑制核因子-κB蛋白的表达。以上各项指标均有一定量效关系。结论：紫草素可以通过抑制过度活化的T淋巴细胞功能从而发挥免疫调控作用，为紫草素治疗银屑病的作用机制及应用前景提供实验依据。 |
| English Summary: |
| To study the effects of shikonin,which is a kind of monomer of Chinese traditional herbs with cooling blood and detoxification,on the function of abnormally activated T lymphocytes and related signal transduction pathways in the pathological state of psoriasis in vitro cell model.Methods:Jurkat E6-1 T lymphocytes were activated with phorbol ester and ionomycin,and shikonin at a concentration of 0.5-2 μg/mL was applied to the cells.T lymphocyte proliferation ability was detected by CCK-8 method.The flow cytometry was used to detect the expression of CD69 and the change of intracellular free calcium concentration (［Ca2+］i).The enzyme-linked immunosorbent assay (ELISA) was used to detect the release of interleukin-2 (IL-2),interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α); q-PCR and Western blot were used to observe the effects of nuclear transcription factor mRNA and protein expression.Results:Different concentrations of shikonin can significantly inhibit cell proliferation,CD69 expression and Th1 cytokine secretion; both can effectively reduce the content of ［Ca2+］i and protein kinase C (PKC) phosphorylated protein,significantly reduce nuclear transcription factor NF-The expression of AT mRNA down-regulates the mRNA and protein expression of c-Jun and inhibit the expression of NF-κB protein.All the above indicators had a certain dose-effect relationship.Conclusion:Shikonin can exert immunoregulatory effects by inhibiting the function of over-activated T lymphocytes,and provide experimental evidence for the mechanism and application prospect of shikonin in the treatment of psoriasis. |
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