世界中医药

作者	单位
林伟明1，王子晨2，吴文敬1，费文婷2，张建军2，熊平1	1 华南农业大学制药工程系，广州，510640； 2 北京中医药大学中医学院，北京，100029

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中文摘要:

目的：评价南美草药阿萨伊的寒热药性。方法：采用MTT细胞增殖抑制试验对比考察阿萨伊、黄柏对细胞株HepG2和Hep3B增殖的影响；倒置相差显微镜对比观察二者对2种细胞株形态特征的影响；台盼蓝染液排斥试验对比分析二者的细胞毒性作用；分光光度法测定阿萨伊处理后的HepG2和Hep3B细胞NADH含量和NADH/NAD+比值的变化，分析其对2种细胞能量代谢的影响。结果：在受试浓度范围内，阿萨伊、黄柏对2种细胞株的生长增殖和能量代谢均呈现不同程度的抑制作用。MTT试验中，阿萨伊浓度≤0.5 μg/mL和≥10 μg/mL时对2种细胞产生增殖抑制效应，差异有统计学意义（P<0.05；P<0.01），且与黄柏作用相似；镜检下细胞的生长疏松程度与上述结果一致，阿萨伊使细胞颗粒感减弱，扁平呈不规则多边形铺展，与黄柏作用相似；台盼蓝染色结果显示各浓度阿萨伊对细胞无明显毒性，亦与黄柏作用相似；阿萨伊对2种细胞胞内能量代谢指标NADH含量、NADH/NAD+比值均有下调作用，差异有统计学意义（P<0.05；P<0.01）。结论：阿萨伊作用于细胞株HepG2和Hep3B后与黄柏作用相似，能够降低能量代谢，其药性偏寒凉。

English Summary:

To evaluate the hot/cold characteristics of Aaí.Methods:MTT cell proliferation inhibition assay was performed to study Aaí's effect on cell proliferation.Inverted phase contrast microscopy was applied to study Aaí's effect on cell morphological features.Trypan Blue staining exclusion assay was used to compare and analyze Aaí's cytotoxicity.A further determination of cellular NADH level and NADH/NAD+ ratio via spectrophotometry was performed to analyze Aaí's effect on cell energy metabolism.Results:Within the range of preset concentrations,Aaí and Cortex Phellodendri demonstrated divergently inhibitive effects on cell growth,proliferation and energy metabolism of the two hepatic cell strains.In the MTT assay,Aaí showed significant (P<0.05) or highly significant (P<0.01) inhibitive effect on cell proliferation at concentrations≤0.5 μg/mL and≥10 μg/mL.Microscopic examination showed corresponding results of lower cell density,attenuated granularity,and more outstretched cells in irregular polygonal shape could be observed.Trypan Blue staining assay showed that any concentration of Aaí had no obvious toxicity to the cells,which was similar to Cortex Phellodendri.Aaí with any preset concentrations was also found to be significantly (P<0.05) or highly significantly (P<0.01) reducing intracellular NADH level as well as NADH/NAD+ ratio.Conclusion:The effects of Aaí on cell lines HepG2 and Hep3B are similar to that of Cortex Phellodendri Chinensis,and can reduce energy metabolism.This study proves that the medicinal property of Aaí is cold or cool.

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