| 引用本文:王敏1，马全涛1，李亚琪1，柳辰玥1，张驰1，邱敏懿1，张彩娟1，王停2，赵保胜2，李红燕3.桑叶对2型糖尿病大鼠肝脏Toll样受体及其下游信号元件基因表达的影响[J].世界中医药,2018,(12):. |
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| 桑叶对2型糖尿病大鼠肝脏Toll样受体及其下游信号元件基因表达的影响 |
| Effects of Folium Mori on the Gene Expression of TLRS and its Downstream Signaling Elements in Liver of T2DM Rats |
| 投稿时间：2018-04-08 |
| DOI：10.3969/j.issn.1673-7202.2018.12.037 |
| 中文关键词: 桑叶 2型糖尿病 大鼠 肝脏 胰岛素抵抗 炎性反应 Toll样受体 mRNA |
| English Keywords:Folium Mori Type 2 diabetic mellitus Rat Liver information retrieval inflammation TLRs mRNA |
| 基金项目:国家自然科学基金项目（81773960） |
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| 中文摘要: |
| 目的：观察桑叶降糖作用，分析桑叶对2型糖尿病(T2DM)大鼠肝脏Toll样受体及其下游信号元件基因表达的影响，初步探讨其降糖机制。方法：选取SPF级雄性Wistar大鼠46只，高脂饲料饲喂8周后，腹腔注射链脲佐菌素（STZ），以制备T2DM模型。根据血糖值将模型大鼠随机分成模型组、阳性药二甲双胍(0.2 g/kg)组和桑叶高、低剂量(3 g/kg,1 g/kg)组，每组12只，另取10只大鼠为正常对照组。灌胃给药，持续12周，每周测其空腹血糖，并于第11周时进行OGTT和ITT实验。以上实验结束后免疫吸附试验（ELISA）试剂盒测定血清中胰岛素、IL-6、肿瘤坏死因子-（TNF-）和C反应蛋白（CRP）水平，实时PCR法测定大鼠肝脏TLRs及MyD88、TRAF-6、核因子-B mRNA水平。结果：大鼠给药桑叶后，血糖值降低，饮水量明显减少，差异有统计学意义（P<0.05），糖耐量得到显著改善，血清中IL-6、CRP水平明显降低，差异有统计学意义（P<0.05），TLR7、TLR8、TLR9以及其下游信号元件TRAF-6、MyD88、核因子-B mRNA相对表达均较模型组明显降低，差异有统计学意义（P<0.05）。结论：桑叶降糖可能与其抑制肝脏组织TLRs及其下游信号表达，降低炎性反应有关。 |
| English Summary: |
| To observe the hypoglycemic effect of Folium Mori，and analyze its effects on the gene expression of toll-like receptor and its downstream signaling element in the liver from type 2 diabetic rats，and to explore its action mechanismo of hypoglycemic.Methods:The rats were fed with high-fat fodder for 8 weeks，and then were given intraperitoneal injection (ig) with streptozotocin (STZ) to establish the T2DM rats model.The diabetic rats were divided into model group，metformin (0.2 g/kg) group，Folium Mori high and low dose groups (3 g/kg，1 g/kg)，with 12 rats in each group，and another 10 rats were selected as the normal control group.The drugs were given by ig administration for 12 weeks，and the fasting blood glucose was detected every week.Oral glucose tolerance test (OGTT) and insulin tolerance test (ITT) were conducted in the mid and later phases of experiment.After the experiment，serum insulin，TNF-，IL-6 and CRP were detected，and the expression level of TLRs and MyD88，TRAF-6，NF-B in the liver were detected by real-time PCR method.Results:After the intervention by Folium Mori，the amount of blood glucose and water intake were decreased significantly (P<0.05 or P<0.01)，the glucose tolerance was improved，the contents of IL-6 and CRP in serum were decreased significantly (P<0.05).The expressions of TLR7，TLR8，TLR9 and TRAF-6，MyD88，NF-B were decreased obviously (P<0.05 or P<0.01).Conclusion:Folium Mori can ameliorate diabetes，and its mechanism may be by inhibiting the expression of TLRs and its downstream signal in liver tissue. |
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