| On the level of compound,prescription medicine,extract and index components,to study the effects of classic Mongolia medicine prescription Chagan Decoction on rat spleen lymphocyte proliferation and cell factor of IL-2 and IFN-γ.Methods:CCK-8 method was used to test the effect of different degree of drug on rat lymphocyte proliferation.The content of IL-2 and IFN-γ were tested by ELISA method.Different polar extracts of Chagan Decoction,4 composition medicinal materials including Inulae Radix,Sophorae Flavescentis Radix,Rubi Ramulus,Kaempferiae Rhizoma,as well as 6 index components,such as alantolactone,isoalantolactone,matrine,oxymatrine,ethyl cinnamate and ethyl-p-methoxycinnamate,were used for the evaluation of lymphocyte proliferation activity.Results:The aqueous extracts of Inulae Radix,Sophorae Flavescentis Radix,Rubi Ramulus,Kaempferiae Rhizoma could promote spleen proliferation lymphocyte in different degrees,and induce secretion of IL-2 and IFN-γ.However,compound,extracts of compound medicinal materials and index component did not show any proliferation effect.Conclusion:The aqueous extracts of Chagan Decoction and compound medicinal materials both could promote the spleen proliferation lymphocyte and play a role in immune regulation through IL-2 and IFN-γ. |
