To improve the depression rat model with neurotransmitters disorder within brain.Methods:According to the results of the sucrose consumption test,the healthy rats were randomly divided into normal control group,original model group and improved model group.Without installing the injection tube,the improved model group was injected SCH23390 into the left hippocampus every 2 days and 4 times in total,the dose was 8 μg/μL.The original model group was placed the injection tube.Through the tube,SCH23390 was given into the left hippocampus at the dose of 2 μg/μL.The medicine was given for 2 days,followed by a one-day rest,and 6 times totally.2 weeks after the modeling,the improved effect was evaluated based on the difference of weight and behavior test (sucrose consumption and the open-field) of different groups.And the athological feature was observed by comparing the change of the content of the 5-HT,5-HIAA,NE,DA and AchE in the left and right hippocampus,as well as in the cerebral cortical.Results:2 weeks after modeling,the weight and the open-field results of the improved model group and the original model group significantly decreased compared to the normal control group (P<0.01),and the sucrose-preference rate decreasing was also significant (P<0.05).Compared with the original model group,the improved model group had no significant difference (P>0.05).It demonstrated that the improved method could make the rats suffer from depression,and the impact was as same as the original.Compared with the normal control group,the level of 5-HT and NE increased significantly and 5-HIAA decreased significantly (P<0.01) in the bilateral hippocampus.The decreasing of DA and the increasing of AchE had no statistical significance (P>0.05).In the cerebral cortical,the content of 5-HT,NE and AchE increased,the content of 5-HIAA and DA decreased,the change was not statically significant (P>0.05).It showed that the improved model group rates had the pathological features of the depression.Conclusion:The modified modeling approach of establishing the depression rat model was to inject the SCH23390 into the left hippocampus of the rat.The dose was 8 μg/μL and the frequency was once every 2 days and 4 times in total.By observation of validity and pathological features,the effectiveness of the improved approach was demonstrated.Furthermore,the modeling time was reduced by cancelling the procedure of the tube installation,and the model could be tested by the forced swimming test or the water maze,which achieved the goal of improvement.