| 引用本文:段玉红1，高泓2，苏虹霞1.三黄汤治疗2型糖尿病的分子机制研究[J].世界中医药,2018,(12):. |
|
| 三黄汤治疗2型糖尿病的分子机制研究 |
| Molecular Mechanism of Sanhuang Decoction in Treating Type 2 Diabetes Mellitus |
| 投稿时间：2018-02-22 |
| DOI：10.3969/j.issn.1673-7202.2018.12.052 |
| 中文关键词: 三黄汤 化学成分 2型糖尿病 分子对接 网络药理学 分子功能 网络模型 蛋白相互作用 |
| English Keywords:Sanhuang Decoction Chemical composition Type 2 diabetes mellitus Molecular docking Network pharmacology Molecular function Network model Protein interaction |
| 基金项目:国家自然科学基金项目(81774304) |
|
| 摘要点击次数: 667 |
| 全文下载次数: 637 |
| 中文摘要: |
| 目的:探讨基于网络药理及分子对接明确三黄汤发挥降糖、降脂的分子机制。方法：选取CTD筛选2型糖尿病的靶标基因。基于网络药理学平台筛选三黄汤发挥作用的有效化学成分，通过DAVID 6.8对靶标基因进行分子功能分析，同时通过STRING进行基因相互作用分析，筛选2型糖尿病关键靶标基因，基于分子对接技术对关键靶标进行验证。并采用Cytoscape 3.5.1软件构建三黄汤化学成分-关键基因网络模型。结果:三黄汤有效化学成分14个，2型糖尿病靶标基因30个。关键靶标基因7个（NFKB1A，BCL2，TNF，RELA，TGFB1，PPARG，HMOX1），分子对接发现三黄汤化学成分与HMOX1，BCL2，PPAR-γ有很好的结合作用。结论:初步阐释三黄汤降糖、降脂的分子机制，为进一步研究三黄汤治疗2型糖尿病靶标基因的选取提供理论指导。 |
| English Summary: |
| To explore the molecular mechanism of Sanhuang Decoction in treating type 2 diabetes mellitus (T2DM) based on network pharmacology and molecular docking.Methods:The effective chemical constituents of Sanhuang Decoction were screened based on the network pharmacology platform，and the target gene of type 2 diabetes was screened by CTD.The molecular function of potential targets were analyzed by DAVID 6.8.At the same time，STRING was used for gene interaction analysis to screen key target genes of type 2 diabetes，and the key targets were verified based on molecular docking technology.The Cytoscape 3.5.1 software was used to construct the network model of Sanhuang Decoction chemical composition-key gene.Results:There were 14 effective chemical composition of Sanhuang Decoction，30 corresponding target genes of type 2 diabetes，7 key target genes (NFKB1A，BCL2，TNF，RELA，TGFB1，PPARG，HMOX1) in Sanghuang Decoction.Through molecular docking，it was found that the chemical constituents of Sanhuang Decoction had a good binding effect with HMOX1，BCL2 and PPARγ.Conclusion:The molecular mechanism of Sanhuang Decoction for reducing blood sugar and lowering lipid was preliminarily explained，which provided theoretical guidance for further study on the selection of Sanhuang Decoction for the treatment of type 2 diabetes target genes. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
