五灵脂炮制前后指纹图谱及含量测定比较
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2016年国家中医药管理局中药标准化行动计划(ZYBZH-C-HUB-20)——小金胶囊标准化建设


Comparison of Fingerprint and Content Test of Faeces Trogopterori before and after Processing
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    摘要:

    目的:建立五灵脂及其炮制品指纹图谱,并对主要指标成分穗花杉双黄酮和扁柏双黄酮进行定量测定。方法:采用HPLC色谱法,Agilent extend-C18柱(4.6 mm×250 mm,5 μm),流动相为乙腈-0.1%甲酸,梯度洗脱,柱温30 ℃,检测波长335 nm,建立五灵脂及其炮制品指纹图谱,采用指纹相似度软件进行分析,并对2个主要色谱峰进行含量分析。结果:醋五灵脂、酒五灵脂、清炒五灵脂与五灵脂生品指纹图谱和含量测定结果基本一致,炭炒五灵脂较五灵脂生品新增2个峰,且穗花杉双黄酮和扁柏双黄酮含量相对减少。结论:所建立的指纹图谱精密度、重复性、稳定性良好,含量测定方法准确率达到要求,对五灵脂及其炮制品的质量评价和炮制原理研究有提供依据。

    Abstract:

    To establish the fingerprint of Faeces Trogopterori and its processed products,and to quantitative analyze the amentoflavone and hinokiflavone.Methods:HPLC (High Performance Liquid Chromatography),Agilent extend-C18 column (4.6 mm×250 mm,5 μm),mobile phase of acetonitrile-0.1% formic acid,gradient elution,column temperature 30 ℃,and detection wavelength 335 nm were established for a fingerprint of Faeces Trogopterori and its processed products.The fingerprints were analyzed by fingerprint similarity software,as well as the contents of two major chromatographic peaks.Results:The fingerprints and content results of vinegar Faeces Trogopterori,wined Faeces Trogopterori,fried Faeces Trogopterori and Faeces Trogopterori were basically the same.The charcoally fried Faeces Trogopterori added 2 peaks to vinegar Faeces Trogopterori,and the contents of amentoflavone and hinokiflavone were relatively reduced.Conclusion:The precision,repeatability and stability of the established fingerprints were good,and the accuracy of the content determination method met the analysis requirements.It provided a basis for the quality evaluation of Faeces Trogopterori processed products and its processing principle.

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陈莹,聂晶,龙小艳,向阳,黄志军.五灵脂炮制前后指纹图谱及含量测定比较[J].世界中医药,2018,(12).

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  • 收稿日期:2018-11-13
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  • 在线发布日期: 2018-12-24
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