世界中医药

作者	单位
陈莹1,3，聂晶3，龙小艳1,3，向阳2，黄志军1	1 武汉理工大学,武汉,430070； 2 健民药业集团股份有限公司,武汉,430052； 3 湖北省药品监督检验研究院,武汉,430075

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中文摘要:

目的：建立五灵脂及其炮制品指纹图谱，并对主要指标成分穗花杉双黄酮和扁柏双黄酮进行定量测定。方法：采用HPLC色谱法，Agilent extend-C18柱(4.6 mm×250 mm，5 μm)，流动相为乙腈-0.1%甲酸，梯度洗脱，柱温30 ℃，检测波长335 nm，建立五灵脂及其炮制品指纹图谱，采用指纹相似度软件进行分析，并对2个主要色谱峰进行含量分析。结果：醋五灵脂、酒五灵脂、清炒五灵脂与五灵脂生品指纹图谱和含量测定结果基本一致，炭炒五灵脂较五灵脂生品新增2个峰，且穗花杉双黄酮和扁柏双黄酮含量相对减少。结论：所建立的指纹图谱精密度、重复性、稳定性良好，含量测定方法准确率达到要求，对五灵脂及其炮制品的质量评价和炮制原理研究有提供依据。

English Summary:

To establish the fingerprint of Faeces Trogopterori and its processed products,and to quantitative analyze the amentoflavone and hinokiflavone.Methods:HPLC (High Performance Liquid Chromatography),Agilent extend-C18 column (4.6 mm×250 mm,5 μm),mobile phase of acetonitrile-0.1% formic acid,gradient elution,column temperature 30 ℃,and detection wavelength 335 nm were established for a fingerprint of Faeces Trogopterori and its processed products.The fingerprints were analyzed by fingerprint similarity software,as well as the contents of two major chromatographic peaks.Results:The fingerprints and content results of vinegar Faeces Trogopterori,wined Faeces Trogopterori,fried Faeces Trogopterori and Faeces Trogopterori were basically the same.The charcoally fried Faeces Trogopterori added 2 peaks to vinegar Faeces Trogopterori,and the contents of amentoflavone and hinokiflavone were relatively reduced.Conclusion:The precision,repeatability and stability of the established fingerprints were good,and the accuracy of the content determination method met the analysis requirements.It provided a basis for the quality evaluation of Faeces Trogopterori processed products and its processing principle.

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