To analyze the content of forsythia and baicalin in Fufang Yuxingcao capsules by high performance liquid chromatography(HPLC)fingerprint.Methods:The Fufang Yuxingcao capsule test solution and phillyrin and baicalin control solution were prepared.Through literature search and pre experiment,HPLC fingerprint was optimized.The chromatographic conditions were ACQUITY-BEHP-henyl-C18 protection column(5 μm).Indoor temperature was column temperature.Injection volume was 20 μL.Acetonitrile was mobile phase A,and water+0.02 % phosphoric acid was mobile phase B.Flow rate was 1.0 mL/min:Detection wavelength was 327 nm.Linear regression,precision,repeatability,stability,specificity test and sample recovery test were performed to observe the rigor stringency of HPLC method.The contents of phillyrin and baicalin in the samples were detected.Results:1)HPLC chromatography was applied to the specific test of Fructus Forsythiae and Radix Scutellariae in Fufang Yuxingcao capsule,which had good specificity.2)Forsyth regression equation Y=98009X-86650,r=0.9992(n=5),and the linearity of phillyrin control in 3.23~103.02 μg/mL was good; Baicalin regression equation Y=212284X+108806,r=0.9998(n=5),and the linearity of baicalin control in 2.74~87.89 μg/mL was good.3)Identification of forsythin and baicalin by 365 nm showed that there was no interference between forsythin and baicalin in the location of negative controls.4)Samples 1:0.51 mg of phillyrin,RSD 1.72%; 14.6 mg of baicalin,RSD 4.95%.Sample 2:0.50 mg phillyrin,RSD 1.69%; 14.3 mg baicalin,RSD 4.92%.There was no significantly difference between phillyrin and baicalin in Fufang Yuxingcao capsule samples(P>0.05).Conclusion:The quality control,stability and specificity of the Fufang Yuxingcao capsule sample are good.