| To study the fingerprint of Xiaoyao Pill on the basis of adulteration test method.Methods:Powder of Xiaoyao Pill was submerged for prepartion,and mixed control solution and the test solution was prepared.The precise of Xiaoyao Pill fingerprint method was tested through the specificity test,linear correlation analysis,precision,repeatability and stability test and recovery test.The menthol and carvone in the sample was tested by gas chromatography(GC),gas chromatography-mass spectrometry(GC-MS),and adulteration detection method was established.Results:1)The test results of specificity,linearity,precision,repeatability,stability and sample recovery were all good.2)Carvone was not detected in samples of Xiaoyao Pill from A factory,and detected in the samples from B and C factory.PA of carvone in samples from C factory was higher than that of B factory.3)According to GC-MS chromatogram,the results showed that GC-MS characteristic peaks of menthol and carvone were m/z 41,55,71,81,95 and 39 m/z,54,82,93,108 using positive ion mode.Conclusion:The fingerprint detection of Xiaoyao Pill on the basis of adulteration test method can effectively control drug quality. |
