世界中医药

作者	单位
赵崇妍1，杨芳1，屈青松1，刘自尧1，高鹏飞2，史新元2	1 北京中医药大学生命科学学院,北京,102488； 2 北京中医药大学中药学院，北京,102488

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中文摘要:

目的：建立蝉拟青霉/黄芪药材、黄芪药渣双向发酵体系，探究发酵过程中有效成分含量变化。方法：选取正交设计法对接种量、发酵温度、湿度进行优化，并采用硫酸-苯酚比色法、NaNO2-Al(NO3)3比色法及香草醛-高氯酸氧化法对发酵黄芪及黄芪药渣菌质中的多糖、总黄酮、总皂苷的含量进行测定。结果：药材菌质最优发酵条件为温度26 ℃，湿度90%，接种比例3 mL/5 g，药渣菌质最优发酵条件为温度26 ℃，湿度80%，接种比例3 mL/5 g；经发酵后，黄芪药材菌质中多糖、总皂苷含量略减少，而黄酮含量有所增加；黄芪药渣菌质中黄酮、皂苷含量均增加。结论：蝉拟青霉可对黄芪药材和药渣基质进行发酵，且发酵后菌质中有效成分的得率显著增加，增强了黄芪药材的药用价值，并为药渣的处理提供了新的思路。

English Summary:

To establish a bidirectional fermentation system of Paecilomyces cicadae/Astragalus membranaceus and Astragalus membranaceus residue，and to explore the content change of active ingredients in fermentation process.Methods:The inoculation amount，temperature and humidity were optimized by orthogonal design method，and the contents of polysaccharide，total flavonoids and total saponins were determined by sulfuric acid-phenol colorimetry，NaNO2-Al (NO3)3 colorimetry and vanillin-perchloric acid oxidation method.Results:The optimum fermentation conditions of medicinal fungus were temperature 26 ℃，humidity 90 %，inoculation ratio 3 mL/5 g.The optimal fermentation conditions of the residues of the fungus were temperature 26 ℃，humidity 80%，inoculation ratio 3 mL/5 g.The contents of polysaccharides and total saponins in Astragalus membranaceus were slightly reduced，while flavonoids content increased.The contents of polysaccharides，flavonoids and saponins in Astragalus membranaceus residue were increased.Conclusion:Paecilomyces cicadae can ferment Astragalus membranaceus medicinal materials and Astragalus residue，and the yield of effective components increased significantly after fermentation，which enhanced the medicinal value of Astragalus membranaceus，and provided a new idea for the disposal of residues.

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