To simultaneous determinate 7 antitumor activity Daphne-type diterpene in Daphne genkwa using a HPLC method (yuanhuadine (1),yuanhuafine (2),genkwadaphnine (3),yuanhuacine (2),yuanhuahine (5),yuanhualine (6),isoyuanhuadine (7)).Methods:Daphne genkwa was extracted with 75% ethanol solution; the selection of column was ZorbaxEelipse SB-C18 (250 mm×4.6 mm,5 m); mobile phase was acetonitrile-0.1% acetic acid water; detection wavelength was 230 nm; column temperature was 40 degrees centigrade; the flow rate was 0.8 mL/min; sample volume was 10 μL; elution gradient was gradient elution system using solvent A (acetonitrile),B (water,0.1% acetic acid).Results:All of the 7 calibration curves mentioned above showed good linearity (r ≥ 0.9941),the average recovery rate was 98.1%-104.5%.The contents of 7 components in Daphne Genkwa indifferent growing environments were different.Conclusion:This method can be used for the determination of Daphne-type diterpene in Daphne genkwa.