Mycotoxins are secondary metabolites produced by certain fungi in traditional Chinese medicine (TCM)，which generally don't show acute toxicity but have strong accumulation.Traditional Chinese medicines are contaminated or potentially polluted by mycotoxins.In order to improve the effect of traditional Chinese medicine matrix on mycotoxin detection，to improve the sensitivity and accuracy of mycotoxin detection，and to ensure human health and reduce the economic loss caused by mycotoxins，this paper summarizes the extraction and purification methods of mycotoxin from TCM which mainly include high-speed homogenization extraction，stirring extraction，ultrasonic extraction，oscillation extraction and immunoaffinity column (ICA) purification，solid phase extraction column (SPE) purification and QuEChERS purification.Some expectation of the methods of mycotoxin pretreatment was brought forward in this article for providing reference for peers at home and abroad.