| 引用本文:吴琪1，2，吴雅韵1，2，韦世杰1，3，余玉英1，3，陈远云1，4，荣向路1，2，3，4，郭姣1，2，3，4.基于气相色谱-质谱的脂肪萎缩小鼠血浆代谢组学研究[J].世界中医药,2019,(01):. |
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| 基于气相色谱-质谱的脂肪萎缩小鼠血浆代谢组学研究 |
| Metabolite in Plasma of aP2-nSREBP-1c Mice with Lipoatrophy Using GC-MS Technology |
| 投稿时间：2018-12-10 |
| DOI：10.3969/j.issn.1673-7202.2019.01.009 |
| 中文关键词: 脂肪萎缩 气相色谱-质谱联用 代谢组学 |
| English Keywords:Lipoatrophy Gas chromatography tandem mass spectrometry Metabonomics |
| 基金项目:国家自然科学基金重点项目（81530102,81830113）；广东省科技计划项目（2017B050504005）；广州市科技计划项目（民生科技攻关计划）（201803010069）；广东省科技厅实验室建设项目（2017B030314064）；广东省科技厅国际合作基地建设项目(2016B050501003) |
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| 摘要点击次数: 1117 |
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| 中文摘要: |
| 目的：脂肪萎缩是引起糖脂代谢紊乱的重要因素之一。通过气相色谱-质谱联用（GC-MS）技术分析aP2-nSREBP-1c脂肪萎缩小鼠血浆代谢谱的变化，研究与其密切相关的差异代谢物与代谢通路，探讨其生物学基础。方法：应用GC-MS技术对6只同窝野生型小鼠和6只转基因小鼠血浆样品进行检测，对检测结果进行多元统计分析，筛选出显著差异的代谢物，借助MetaboAnalyst分析代谢通路。结果：与野生型（wild-type，WT）小鼠比较，转基因（transgenic，TG）小鼠血浆中乳酸、L-缬氨酸、3-羟基丁酸、尿素、d-半乳糖、D-阿洛糖、硬脂酸、棕榈酸、肌醇、油酸、11-十八碳烯酸含量增加。代谢通路富集分析发现3条脂肪萎缩相关通路。结论：通过对aP2-nSREBP-1c小鼠血浆代谢组学分析，发现缬氨酸、亮氨酸和异亮氨酸的生物合成，丙酮酸代谢，磷酸肌醇代谢途径与脂肪萎缩相关，为阐明糖脂代谢性疾病的发病机制提供了代谢组学依据。 |
| English Summary: |
| To analyze the changes in plasma metabolism using gas chromatography-mass spectrometry (GC-MS) technology of aP2-nSREBP-1c mice with lipoatrophy. Study the differential metabolites and metabolic pathways that are closely related to lipoatrophic and explore its molecular mechanisms. Methods:Plasma samples of 6 wild-type mice in the in the control group and 6 transgene-type mice in the model group were detected using GC-MS. The result was analyzed by using multivariate statistical analysis，the metabolites were screened out which have significant difference. Results:Compared with the control group (wild-type，WT)，the plasma levels of D-(-)-Lactic acid，L-Valine，(R)-3-Hydroxybutyric acid，Urea，d-Galactose，D-Allose，Stearic acid，Palmitic acid，Myo-Inositol，Oleic acid，11-Octadecenoic acid in the model group (transgene-type，TG) increased. Pathway enrichment analysis displayed that these metabolites were mainly involved in 3 metabolic pathways which were closely related to lipoatrophy. Conclusion:Through plasma metabonomics analysis of aP2-nSREBP-1c rats，metabolic information highly associate with lipoatrophy. The finding is helpful to provide a basis for understanding the molecular mechanisms of the disease. |
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