世界中医药

目的:探讨八味茵术颗粒剂中6,7-二甲氧基香豆素、白术内酯Ⅲ、白术内酯Ⅰ的含量测定方法。方法：选取DIKMA Diamonsil C18（2）色谱柱（4.6 mm×250 mm，5 μm），柱温30 ℃，DAD检测（200~400 nm）：6,7-二甲氧基香豆素的检测波长为340 nm，白术内酯Ⅲ的检测波长为220 nm，白术内酯Ⅰ的检测波长为275 nm。进样量为5 μL，流动相为水（A）-乙腈（B）梯度洗脱，流速为1 mL/min。结果：6,7-二甲氧基香豆素在0.1~100.0 μg/mL范围内线性关系良好（R2=1.0000）、白术内酯Ⅲ在0.1~100.0 μg/mL范围内线性关系良好（R2=1.0000）、白术内酯Ⅰ在0.1~100.0 μg/mL范围内线性关系良好（R2=1.0000），其精密度、稳定性、加样回收率均符合含量测定要求。结论：本方法简单易行，准确可靠，可有效用于八味茵术颗粒剂的质量控制。

English Summary:

To establish the HPLC method for determining the contents of 6,7-dimethoxycoumarin，atractylenolide III and atractylenolide I in Bawei Yinzhu Granules. Methods:The DIKMA Diamonsil C18 (2) (4.6 mm×250 mm，5 μm) column was adopted to separate the sample at the column temperature of 30 ℃. The detection wavelength was set between 200 nm to 400 nm. The detection wavelength of 6,7-dimethoxycoumarin，atractylenolide III and atractylenolide I were 340 nm，220 nm and 275 nm respectively. The injection volume was 5 μL. The mobile phase consisted of water-acetonitrile with a gradient elution at a flow rate of 1.0 mL/min. Results:The calibration curves of were linear in the ranges of 0.1-100.0 μg/mL for 6,7-dimethoxycoumarin (R2=1.0000); the calibration curves were linear in the ranges of 0.1-100.0 μg/mL for atractylenolide III (R2=1.0000); and the calibration curves were linear in the ranges of 0.1-100.0 μg/mL for atractylenolide I (R2＝1.0000). The precision，accuracy and recovery met the determination requirements. Conclusion:The method is simple and feasible，and thus can be used for the quality control of Bawei Yinzhu Granules.

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