| 引用本文:车晓平1，王天园2，王宏2，韩旭阳2，彭冰2，曾祖平2.狼疮合剂的质量控制研究[J].世界中医药,2019,(01):. |
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| 狼疮合剂的质量控制研究 |
| Study on Quality Control of Lupus Mixture |
| 投稿时间：2016-07-05 |
| DOI：10.3969/j.issn.1673-7202.2019.01.015 |
| 中文关键词: 狼疮合剂 龙胆苦苷 高效液相色谱法 薄层色谱法 |
| English Keywords:Lupus mixture Gentiopicroside High performance liquid chromatography Thin-layer chromatography |
| 基金项目: |
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| 中文摘要: |
| 目的：建立狼疮合剂的质量控制标准。方法：对狼疮合剂中淫羊藿、鸡血藤、黄芪、白术进行薄层色谱法定性鉴别，对君药秦艽含有的龙胆苦苷进行高效液相色谱法含量测定。结果：薄层色谱图特征斑点清晰、分离较好，阴性对照无干扰；龙胆苦苷在0.202 5～3.24 μg范围内与峰面积积分值呈良好的线性关系，平均加样回收率为102.56％，RSD＝1.57％（n=6），3批狼疮合剂中龙胆苦苷平均含量为1.70 mg/mL。结论：本法准确、灵敏,重复性好，为狼疮合剂的质量控制提供了依据。 |
| English Summary: |
| To establish quality control standards of lupus mixture. Methods:Herba Epimedii，Caulis Spatholobi，Radix Astragali seu Hedysari，Rhizoma Atractylodis Macrocephalae of lupus mixture were identified by TLC (Thin-Layer Chromatography). Gentiopicroside of lupus mixture was determined by HPLC (High Performance Liquid Chromatography). Results:Characteristics of TLC spots were clear with better separation，and negative control had no interference. It was linear in the range of 0.202 5-3.24 μg，and the rate of average recovery was 102.56% with RSD of 1.57％(n=6) for Gentiopicroside. Average concentrations of Gentiopicroside in three batches of Lupus mixture were 1.70 mg/mL. Conclusion:The method is accurate，delicacy and reproducible，providing a basis for quality control of lupus mixture. |
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