世界中医药
文章摘要
引用本文:车晓平1,王天园2,王宏2,韩旭阳2,彭冰2,曾祖平2.狼疮合剂的质量控制研究[J].世界中医药,2019,(01):.  
狼疮合剂的质量控制研究
Study on Quality Control of Lupus Mixture
投稿时间:2016-07-05  
DOI:10.3969/j.issn.1673-7202.2019.01.015
中文关键词:  狼疮合剂  龙胆苦苷  高效液相色谱法  薄层色谱法
English Keywords:Lupus mixture  Gentiopicroside  High performance liquid chromatography  Thin-layer chromatography
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作者单位
车晓平1,王天园2,王宏2,韩旭阳2,彭冰2,曾祖平2 1 首都医科大学附属北京中医医院药学部北京100010 2 北京市中医研究所中药室北京100010 
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中文摘要:
      目的:建立狼疮合剂的质量控制标准。方法:对狼疮合剂中淫羊藿、鸡血藤、黄芪、白术进行薄层色谱法定性鉴别,对君药秦艽含有的龙胆苦苷进行高效液相色谱法含量测定。结果:薄层色谱图特征斑点清晰、分离较好,阴性对照无干扰;龙胆苦苷在0.202 5~3.24 μg范围内与峰面积积分值呈良好的线性关系,平均加样回收率为102.56%,RSD=1.57%(n=6),3批狼疮合剂中龙胆苦苷平均含量为1.70 mg/mL。结论:本法准确、灵敏,重复性好,为狼疮合剂的质量控制提供了依据。
English Summary:
      To establish quality control standards of lupus mixture. Methods:Herba Epimedii,Caulis Spatholobi,Radix Astragali seu Hedysari,Rhizoma Atractylodis Macrocephalae of lupus mixture were identified by TLC (Thin-Layer Chromatography). Gentiopicroside of lupus mixture was determined by HPLC (High Performance Liquid Chromatography). Results:Characteristics of TLC spots were clear with better separation,and negative control had no interference. It was linear in the range of 0.202 5-3.24 μg,and the rate of average recovery was 102.56% with RSD of 1.57%(n=6) for Gentiopicroside. Average concentrations of Gentiopicroside in three batches of Lupus mixture were 1.70 mg/mL. Conclusion:The method is accurate,delicacy and reproducible,providing a basis for quality control of lupus mixture.
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