| 引用本文:王首帆，徐宜厚，徐爱琴，朱立宏，秦宗碧，邱百怡.复方黄芩“水膜”外用治疗急性湿疹抗炎和抑制金葡菌的实验研究[J].世界中医药,2019,(01):. |
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| 复方黄芩“水膜”外用治疗急性湿疹抗炎和抑制金葡菌的实验研究 |
| Experimental Study on the External Treatment of Compound Radix Scutellariae “Water Film” of Acute Eczema with Anti-Inflammatory and Inhibiting Staphylococcus Aureus |
| 投稿时间：2017-07-07 |
| DOI：10.3969/j.issn.1673-7202.2019.01.017 |
| 中文关键词: 复方黄芩 “水膜” 急性湿疹 抗炎 金黄色葡萄球菌 |
| English Keywords:Compound Radix Scutellariae Water film Acute eczema Anti inflammation Staphylococcus aureus |
| 基金项目:武汉市卫生和计划生育委员会科研项目（WZ16Z01） |
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| 中文摘要: |
| 目的：探讨复方黄芩“水膜”外用对急性湿疹大鼠皮肤抗炎和抑制金黄色葡萄球菌的作用机制。方法：64只雄性大鼠根据随机数字表法分为复方黄芩“水膜”观察组（黄芩组）、黄蒲洁肤洗液组（黄蒲组）、硼酸液组（硼酸组）和生理盐水组（盐水组）4组,每组16只,鼠右耳予以100%二甲苯致炎后,分别用各组药物湿敷1 h进行干预。检测大鼠右耳肿胀程度；常规HE染色,观察各组每个视野100个表皮细胞中的炎性反应细胞数。采用试管稀释法对4组所用药物作连续对倍稀释,观察体外对金黄色葡萄球菌的抑制情况。结果：1）与盐水组比较,黄芩组、黄蒲组和硼酸组大鼠右耳肿胀度均下降(均P<0.05)，其中黄芩组和硼酸组较黄蒲组显著下降(P<0.05),而黄芩组与硼酸组间比较，差异无统计学意义(P>0.05)。2）与盐水组比较,黄芩组、黄蒲组和硼酸组大鼠右耳表皮细胞中炎性反应细胞数均减少(均P<0.05)，其中黄芩组和硼酸组较黄蒲组显著减少(P<0.05),而黄芩组与硼酸组间差异无统计学意义(P>0.05)。3）黄芩组最低抑菌浓度（MIC）为15.6 mg/mL(1:16)，药物最小杀菌浓度（MBC）为7.8 mg/mL(1:8),高于硼酸组(P<0.05)而低于黄蒲组(P<0.05)。结论：复方黄芩“水膜”外用治疗急性湿疹能有效改善大鼠的肿胀度,具有良好的抗炎和抑制金黄色葡萄球菌的作用。 |
| English Summary: |
| To study the compound Radix Scutellariae “water film” in the treatment of acute eczema with anti-inflammatory and inhibiting Staphylococcus aureus. Methods:A total of 64 male rats were randomly divided into Compound Radix Scutellariae “water film” treatment group，Huang Pu Lotion group，boric acid solution group and normal saline group with 16 in each group. Mice were given 100% xylene induced inflammation respectively，and each group was given wet drug 1 h intervention. The degree of ear swelling in rats was detected. The number of inflammatory cells in 100 epidermal cells of each group was observed by routine HE staining. The drugs of 4 groups were continuously diluted for two times，and the inhibitory effect on Staphylococcus aureus was observed in vitro. Results:1) Compared with the normal saline group，right ear swelling of rats in Compound Radix Scutellariae “water film” group，Huang Pu Lotion group and boric acid group decreased (P<0.05); the Compound Radix Scutellariae “water film” group and boric acid group than in Huang Pu Lotion group decreased significantly (P<0.05)，but no significant difference between Radix Scutellariae group and boric acid group (P>0.05). 2) Compared with the noemal saline group，the number of inflammatory cells of right ear epidermal cells of rats in Radix Scutellariae group，Huang Pu Lotion Group and boric acid group were decreased (P<0.05); the inflammatory cells in Radix Scutellariae group and boric acid group decreased more significantly than in Huang Pu Lotion group (P<0.05),but no significant difference between Radix Scutellariae group and boric acid group (P>0.05). 3) The MIC of Radix Scutellariae group was 15.6 mg/mL (1:16) and MBC was 7.8 mg/mL (1:8)，which was higher than that of boric acid (P<0.05) and lower than that of Huang Pu Lotion group (P<0.05). Conclusion:The treatment of acute eczema with Compound Radix Scutellariae “water film” can improve the swelling degree of rats，and has good anti-inflammatory and inhibitory effect on Staphylococcus aureus. |
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