| 引用本文:张淑静，王玥，周雨玫，陈琳，高誉珊，黄翔，孙燕，郑丰杰，李宇航.跑步运动对小鼠端粒长度和体内氧化抗氧化的影响[J].世界中医药,2019,(02):. |
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| 跑步运动对小鼠端粒长度和体内氧化抗氧化的影响 |
| Effects of Running on Telomere Length and Oxidative Antioxidant Level in Mice |
| 投稿时间：2018-04-19 |
| DOI：10.3969/j.issn.1673-7202.2019.02.016 |
| 中文关键词: 中医养生 跑步运动 端粒 氧化抗氧化水平 氧化损伤 小鼠 过氧化氢酶 氧化物歧化酶 |
| English Keywords:Traditional Chinese medicine regimen Running Telomere Oxidation and antioxidant level Oxidative damage Mice Catalase Oxide dismutase |
| 基金项目:北京中医药大学“经方现代应用关键科学问题的基础研究”创新团队(2011-CXTD-04)；国家自然科学基金项目(81373503) |
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| 中文摘要: |
| 目的：观察跑步运动对小鼠端粒长度和体内氧化抗氧化的影响，探讨中医学“流水不腐，户枢不蠢”运动养生观的现代生物学机制。方法：选取并将8周龄雄性ICR小鼠随机分为3组（未施加运动组，运动1组，运动2组），两运动组按2种不同的运动量,每天跑步，共运动8周后取材。荧光定量PCR的方法检测血液细胞和肝脏组织中基因端粒的长短变化，化学法测定试剂盒检测肝脏组织中还原型胱甘肽（GSH）/氧化型谷胱甘肽（GSSG）比值的变化，总超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、心肌组织中蛋白质羰基和丙二醛（MDA）的水平，用酶联免疫吸附试验（ELISA）试剂盒检测心肌组织中8-异前列腺素F2α（Direct 8-iso-PGF2α8）的水平和8-羟基脱氧鸟苷(8-OHdG)的水平。结果:运动1组和运动2组血液细胞中端粒的长度均长于未施加运动组，差异有统计学意义（P<0.05）；肝脏组织中端粒的长度，虽长于未施加运动组，但差异无统计学意义（P>0.05）。运动1组与未施加运动组比较，小鼠肝组织中GSH/GSSG的比值变大，差异有统计学意义（P<0.05），运动2组与未施加运动组比较，差异无统计学意义（P>0.05）；运动1组和运动2组与未施加运动组比较，肝组织中过氧化氢酶（CAT）的水平升高，差异有统计学意义（P<0.05），3组小鼠肝组织中总超氧化物歧化酶（SOD）水平差异无统计学意义（P>0.05）。运动1组和运动2组与未施加运动组比较，8-OHdG水平、蛋白质羰基水平、异前列腺素F2α水平均降低，差异有统计学意义（P<0.05）。运动1组与未施加运动组比较，丙二醛水平降低，差异有统计学意义（P<0.05），运动2组与未施加运动组比较，差异无统计学意义（P>0.05）。结论：一定强度的运动可以升高抗氧化防御作用，降低氧化损伤，减缓端粒的缩短速度。 |
| English Summary: |
| To observe the effects of running exercise on telomere length and the oxidation and antioxidation level in mice，and to explore the modern biological mechanism of exercise in the traditional Chinese medicine.Methods:Eight-week-old male ICR mice were randomly divided into three groups (no exercise group，exercise group 1，and exercise group 2).The two exercise groups were given different amount of daily running for 8 weeks.The quantitative real-time PCR method was used to detect the change of telomere length in blood cells and liver tissue.The chemical assay kit was used to detect the change of glutathione (GSH)/oxidized glutathione (GSSG) in liver tissue，and to detect the change in total superoxide dismutase (SOD)，catalase (CAT)，protein carbonyl and malondialdehyde (MDA) content in myocardial tissue.The content of 8-isoprostaglandin F2α and 8-hydroxydeoxyguanosine (8-OHdG) in cardiac muscle tissue were detected using the ELISA kits.Results:Compared with the non-exercise group，the length of telomere in the blood cells of exercise group 1 and exercise group 2 was significantly longer than that of the no exercise group (P<0.05 or P<0.01).Although longer than the no exercise group，the length of telomeres in liver was not statistical significant.The ratio of GSH/GSSG in liver tissue of mice in exercise group 1 was higher than that in no exercise group，and there was no significant difference between exercise group 2 and non-exercise exercise group.Compared with the non-exercise group，the contents of catalase (CAT) in the liver tissue were significantly higher in exercise group 1 and exercise group 2，the difference was statistically significant (P<0.05 or P<0.01).No significant difference was found in the total superoxide dismutase (SOD) content in the tissues.Compared with no exercise group，the levels of 8-OHdG，protein carbonyl，and isoprostaglandin F2α in exercise group 1 and exercise 2 group were significantly decreased (P<0.05 or P<0.01).Compared with the no exercise group，the content of malondialdehyde in the exercise group 1 was decreased，and the difference was statistically significant (P<0.05)，while there was no significant difference between the exercise group 2 and the no exercise exercise group.Conclusion:A certain intensity of exercise can increase the antioxidant defenses，reduce oxidative damage，and slow down the telomere's shortening. |
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