世界中医药

目的：观察艾灸对胃荷瘤大鼠外周血中CD4+、CD4+CD25+T淋巴细胞亚群的影响。方法：将50只健康SPF级SD大鼠，雌雄各半，随机分为空白组、假手术组、模型组、艾灸组和红外组5组，每组10只。利用Walker-256细胞来源的实体瘤组织移植胃部制备模型。模型成功后，艾灸组予以悬灸中脘、胃俞等穴位；红外组予以短波红外线照射腹部胃脘及背部胃脘投影区；其余组捆绑束缚。干预20 min/次，1次/d，持续21 d。干预结束后，眼眶采血，流式细胞技术测定外周血中CD4+、CD4+CD25+T淋巴比例。处死动物，测算胸腺指数及肿瘤体积生长抑制率。结果：与空白组比较，模型组动物生存状态变差，胸腺指数增加，外周血中CD4+CD25+T淋巴细胞比例显著增加（P<0.05）；与模型组比较，艾灸组动物艾灸组动物生存状态改善，胸腺指数进一步增加，瘤体内坏死灶较多，血管较少，外周血中CD4+T淋巴细胞比例显著增加（P<0.01），CD4+CD25+T淋巴细胞比例减少（P<0.01）；与红外组比较，艾灸组瘤体内坏死灶更多，血管较少，外周血中CD4+T淋巴细胞比例增加（P<0.01）。结论：艾灸可一定程度改善荷瘤动物生存状态，减少肿瘤细胞转移、促进肿瘤细胞坏死，减少血管增生。这种影响可能与增加CD4+T淋巴细胞，减少CD4+CD25+T淋巴细胞有关。

English Summary:

To observe the effects of moxibustion on CD4+ and CD4+CD25+ T lymphocytes in peripheral blood of rats with gastric cancer. Methods:A total of 50 healthy SPF SD rats(half male and half female)were randomly divided into 5 groups：the blank group，the sham operation group，the model group，the moxibustion group and the infrared group，with 10 rats in each group. Solid tumor tissue derived from Walker-256 cells was transplanted into the stomach to prepare the tumor model. After modeling，moxibustion group and infrared group were treated 20 mins per day for 21 days. After the intervention，the blood from the orbits was collected. And the ratio of CD4+ and CD4+CD25+ T lymphocytes in peripheral blood was tested by flow cytometry. After killing the rats，the thymus index and the growth inhibition rate of the tumor volume were measured. Results:Compared with the blank group，the survival status of the model group was worse，the thymus index increased，and the proportion of CD4+CD25+ T lymphocytes in peripheral blood significantly increased(P<0.05). Compared with the model group，the survival status of the moxibustion group was improved，the thymus index was further increased，more necrosis and less blood vessels were found in the tumors，and the percentage of CD4+ T lymphocytes was increased significantly(P<0.01)，while that of CD4+CD25+ T lymphocyte was decreased(P<0.01). Compared with the infrared group，more necrosis and few blood vessels were found in the tumors，and the proportion of CD4+ T lymphocytes was increased in the moxibustion group(P<0.01). Conclusion:Moxibustion can improve the survival status of the rat with gastric tumor，inhibit tumor metastasis，promote tumor necrosis as well as prevent vessels hyperplasia. These changes may be related to the increasing CD4+ T lymphocytes and the decreasing CD4+CD25+ T lymphocytes.

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