| 引用本文:魏星，谭静，言芳，孙希，孙璐，罗明，阳仁达.隔药饼灸对慢性支气管炎模型大鼠肺组织内p-p38MAPK、ICAM-1的影响[J].世界中医药,2019,(03):. |
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| 隔药饼灸对慢性支气管炎模型大鼠肺组织内p-p38MAPK、ICAM-1的影响 |
| Effects of Cake-separated Moxibustion on p-p38MAPK and ICAM-1 in Rats' Lung Tissue with Chronic Bronchitis |
| 投稿时间：2019-02-01 |
| DOI：10.3969/j.issn.1673-7202.2019.03.009 |
| 中文关键词: 隔药饼灸；慢性支气管炎；中性粒细胞；ICAM-1；p-p38MAPK 大鼠 模型 |
| English Keywords:Cake-separated moxibustion Chronic bronchitis Neutrophil ICAM-1 p-p38MAPK Rat Model |
| 基金项目:湖南省教育厅创新平台项目（16K068）；湖南省大学生创新实验项目（017-132） |
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| 中文摘要: |
| 目的：观察隔药饼灸对慢性支气管炎大鼠p-p38MAPK、ICAM-1的影响。方法：将48只SD大鼠随机分成空白组、模型组、隔药饼灸组、隔纸垫灸组，每组12只。选用改良烟熏法建立大鼠慢性支气管炎模型，干预后，测定肺泡灌洗液中性粒细胞含量，各组大鼠ICAM-1 mRNA选用RT-PCR术测定、ICAM-1及p-p38MAPK蛋白选用Western blot检测。结果：与空白组比较，模型组中性粒细胞、p-p38MAPK、ICAM-1 mRNA及蛋白含量增高显著（P<0.01）。与模型组比较，隔药饼灸组中性粒细胞数目减少（P<0.05），p-p38MAPK、ICAM-1 mRNA及蛋白含量显著降低（P<0.01）。与隔纸垫灸组比较，隔药饼灸组中性粒细胞、p-p38MAPK蛋白、ICAM-1 mRNA及蛋白降低更明显（P<0.05）。结论：隔药饼灸可通过减少p-p38MAPK蛋白含量、降低ICAM-1 mRNA及其蛋白水平，抑制中性粒细胞的释放，以改善炎性反应环境治疗慢性支气管炎。 |
| English Summary: |
| To observe the effects of cake-separated moxibustion on intercellular adhesion molecule-1(ICAM-1)and p-p38MAPK in rats with chronic bronchitis. Methods:A total of 48 Sprague-Dawley(SD)rats were randomly separated into the blank group，model group，cake-separated moxibustion group and paper pad-separated moxibustion team(12 in each group). In addition to the blank group，other 3 groups used modified fumigation to establish the chronic bronchitis models of rats. After intervention，the content of neutrophils in Bronchoalveolar Lavage Fluid(BALF)was measured. ICAM-1 mRNA was detected by RT-PCR，while protein contents of ICAM-1 and p-p38MAPK were detected by Western blot. Results:Compared with the blank group，the contents of neutrophils，p-p38MAPK and ICAM-1 mRNA were obviously increased in the model group(P<0.01). Compared with the model group，the number of neutrophils was decreased(P<0.05)and the contents of p-p38MAPK，ICAM-1 mRNA were significantly decreased(P<0.01). Compared with the paper pad-separated moxibustion group，the contents of neutrophils，p-p38MAPK，ICAM-1 mRNA and protein were significantly decreased in the cake-separated moxibustion group(P<0.05). Conclusion:Cake-separated moxibustion can inhibit the release of neutrophils and significantly reduce the inflammatory reaction，by reducing the contents of p-p38MAPK，ICAM-1 mRNA and protein，so as to treat chronic bronchitis. |
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