To establish a method for the simultaneous determination of 26 kinds of mycotoxins in traditional Chinese medicine Notoginseng Radix et Rhizoma using a QuEChERS pretreatment procedure coupled with ultra-high-performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS).Methods:The samples were pretreated with QuEChERS method，extracted with 15% formic acid in acetonitrile，purified by mixed absorbents，as well as analyzed by UHPLC-MS/MS.Matrix-matched calibration curves and internal standards were used for accurate quantification.Results:Satisfactory recoveries at low，medium and high spiked levels were ranged from 80.1% to 116.2%(n＝5).Good linear relationships were obtained and the correlation coefficients(r2）were greater than 0.99.The limits of detection(LODs，S/N=3）and quantification(LOQs，S/N=10）ranged from 0.05 μg/kg to 6.25 μg/kg and from 0.2 μg/kg to 20.0 μg/kg，respectively.Conclusion:The proposed method is simple，rapid and valuable，which can be a powerful tool for quantitative analysis of the 26 mycotoxins in Notoginseng Radix et Rhizoma.