| 引用本文:王少敏1，杜春晓1，刘贤贤1，刘冠萍2，毛，丹1，梁云飞2，黄晓静1，朱小凤2，陈钶1，季申1.QuEChERS-超高效液相色谱-串联质谱法同时测定三七中26种真菌毒素[J].世界中医药,2019,(04):. |
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| QuEChERS-超高效液相色谱-串联质谱法同时测定三七中26种真菌毒素 |
| Simultaneous Determination of 26 Mycotoxins in Notoginseng Radix et Rhizoma by QuEChERS-Ultra-High-performance Liquid Chromatography-Tandem Mass Spectrometry |
| 投稿时间：2019-03-10 |
| DOI：10.3969/j.issn.1673-7202.2019.04.003 |
| 中文关键词: QuEChERS 真菌毒素 超高效液相色谱-串联质谱法 三七 同时测定 黄曲霉毒素 赭曲霉毒素 恩镰孢菌素 |
| English Keywords:QuEChERS Mycotoxins Ultra-High-performance liquid chromatography-tandem mass spectrometry Notoginseng Radix et Rhizoma Simultaneous Determination aflatoxins Ochratoxins Enniatins |
| 基金项目:国家自然科学基金项目（81603292）；科技部重点研发专项（2017YFC1700800）；国家中药标准化项目（ZYBZH-C-GX-09） |
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| 中文摘要: |
| 目的：采用改良QuEChERS前处理技术，建立了超快速液相色谱-串联质谱(Ultra-High-performance Liquid Chromatography-TandemMass Spectrometry，UHPLC-MS/MS)检测中药三七中26种真菌毒素含量的分析方法。方法：样品用QuEChERS方法进行前处理，经15%甲酸乙腈溶液提取，用混合净化填料净化除杂，采用基质匹配标准曲线结合同位素内标法进行定量分析。结果：在低、中、高3个添加水平下，26种真菌毒素的平均加标回收率为80.1%～116.2%(n=5)，平均回收率范围为26种真菌毒素在各自的线性范围内线性关系良好，相关系数(r2）≥0.99，检出限和定量限分别为0.05～6.25 μg/kg和0.2～20.0 μg/kg。结论：该法简单、快速、实用性强，适用于三七中26种真菌毒素的定量分析。 |
| English Summary: |
| To establish a method for the simultaneous determination of 26 kinds of mycotoxins in traditional Chinese medicine Notoginseng Radix et Rhizoma using a QuEChERS pretreatment procedure coupled with ultra-high-performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS).Methods:The samples were pretreated with QuEChERS method，extracted with 15% formic acid in acetonitrile，purified by mixed absorbents，as well as analyzed by UHPLC-MS/MS.Matrix-matched calibration curves and internal standards were used for accurate quantification.Results:Satisfactory recoveries at low，medium and high spiked levels were ranged from 80.1% to 116.2%(n＝5).Good linear relationships were obtained and the correlation coefficients(r2）were greater than 0.99.The limits of detection(LODs，S/N=3）and quantification(LOQs，S/N=10）ranged from 0.05 μg/kg to 6.25 μg/kg and from 0.2 μg/kg to 20.0 μg/kg，respectively.Conclusion:The proposed method is simple，rapid and valuable，which can be a powerful tool for quantitative analysis of the 26 mycotoxins in Notoginseng Radix et Rhizoma. |
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