| 引用本文:张甦，胡青，孙健，冯睿，于泓，季申.UPLC/MS/MS法定性定量测定中药材中3类27种合成色素[J].世界中医药,2019,(04):. |
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| UPLC/MS/MS法定性定量测定中药材中3类27种合成色素 |
| Qualitative and Quantitative Determination of 27 Synthetic Dyes of 3 Species in Herbs by UPLC/MS/MS |
| 投稿时间：2019-03-10 |
| DOI：10.3969/j.issn.1673-7202.2019.04.007 |
| 中文关键词: 27种 合成色素 脂溶性 酸性 碱性 定性定量 中药材 UPLC/MS/MS |
| English Keywords:27 kinds Artificial dyes Lipophilic,Acidity Basicity Qualitative and quantitative determination Herbs UPLC/MS/MS |
| 基金项目:中国药典2015年版标准提高方法研究项目（中药中常用色素检测方法的研究）；上海市科委技术服务平台（18DZ2292200） |
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| 中文摘要: |
| 目的：采用液相-固相萃取-超高效液相色谱-电喷雾串联质谱联用技术对27种色素建立了准确、灵敏的分析方法。方法：针对脂溶性、酸性和碱性3类色素分别采用不同的制备方法、色谱条件和电喷雾极性，采用4种不同药用部位的代表性药材进行了方法学考察。结果：表明方法线性关系良好（r≥0.997）；脂溶性和碱性色素的检测限为0.03~3.87 ng/g，酸性色素为0.003~1.94 μg/g；准确度和精密度分别为70%～105%和0.9%～6.8%；应用于25个品种80批样品的筛查。结论：所建方法可靠、高效、准确，适用于中药材复杂基质中不同种类色素的测定。 |
| English Summary: |
| To develop a sensitive and accurate method combining liquid and solid phase extraction with ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry to analyze 27 dyes.Methods:Different preparations，chromatography conditions and polarity of ESI were applied for 3 categories of lipophilic，acidic and basic dyes respectively.Four species of herbs representing different parts of plant were selected for methodology observation.Results:The linear correlation coefficient was good(r ≥ 0.997).The LODs of oil soluble and basic dyes were 0.03～3.87 ng/g and acid dyes were 0.003～1.94 μg/g respectively.The accuracies and precisions were 70%～105% and 0.9%～6.8% respectively.Conclusion:After detecting 80 samples of 25 species herbs，this method proved to be reliable，efficient and accurate in the detection of different kinds of dyes in the complex matrix of herbs. |
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