世界中医药

基金项目:国家自然科学基金项目(81473555,81703981)；黑龙江省博士后科研启动金资助项目(LBH-Q16213)；黑龙江省普通本科高等学校青年创新人才培养计划(UNPYSCT-2016077)；黑龙江省教育厅国自然配套项目(2017PT07)；黑龙江中医药大学科研基金项目（201739）

作者	单位
张玉昆，冯月男，卞敬琦，肖洪彬，牛雯颖	黑龙江中医药大学,哈尔滨,150040

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中文摘要:

目的：观察丹参饮对气虚血瘀模型大鼠血小板生物学的影响，从血小板动态变化上探讨丹参饮作用机制。方法：选取SD大鼠40只为研究对象，一次性向气管内注射博莱霉素生理盐水溶液复制气虚血瘀模型,将40只SD大鼠随机分为空白对照组、气虚血瘀模型组、丹参饮高剂量组(3.6 g/kg)、低剂量组(0.9 g/kg)，每组10只。造模后第2天开始灌胃给药，连续21 d，末次给药1 h后5%水合氯醛麻醉，腹主动脉取血，检测全血黏度、血浆黏度、血小板参数、血小板活化黏附、聚集、释放以及纤溶相关指标。结果：丹参饮可显著降低模型大鼠中低切变率下的全血黏度和血浆黏度，差异有统计学意义（P<0.05），可显著降低PDW和MPV血小板相关参数，差异有统计学意义（P<0.05）；可显著升高环磷酸腺苷（cAMP）水平，差异有统计学意义（P<0.05），降低环磷酸鸟苷（cGMP）、β-血小板球蛋白（β-TG）血小板第4因子（PF4）和纤溶酶原激活物抑制剂-1（PAI-1）水平，差异有统计学意义（P<0.05），延长凝血酶原时间（PT）和凝血酶时间（TT），差异有统计学意义（P<0.05）。结论：丹参饮可能主要通过改善血小板聚集、抑制凝血及血小板释放亢进的状态方面达到降低全血黏度，改善气虚血瘀状态。

English Summary:

To observe the effects of Danshen Decoction on the biological indexes of platelets in model rats with blood stasis due to qi deciency, and to explore the mechanism of Danshen Decoction from the dynamic changes of platelets. Methods:A total of 40 SD rats were randomly assigned to blank group, model group, high-dose Danshen Decoction group (3.6 g/kg) and low-dose Danshen Decoction group (0.9 g/kg) with 10 rats in each group after establishing the model of blood stasis due to qi deciency through injecting bleomycin saline solution into trachea at one time. On the second day after establishing model, the medicine was given through intragastric administration for 21 days. After one hour of the last administration, the rats were anesthetized with 5% chloral hydrate and blood was taken from the abdominal aorta. Blood viscosity, plasma viscosity, platelet parameters, platelet activation, adhesion, aggregation, release and related indicators of fibrinolysis were detected. Results:Compared with the model group, Danshen Decoction could significantly reduce the blood viscosity (P<0.05) and plasma viscosity (P<0.05). PDW and MPV could be reduced significantly (P<0.05). The content of cAMP could be increased significantly (P<0.05). The content of cGMP, β-TG, PF4 and PAI-1 could be reduced significantly (P<0.05). And prothrombin time (PT) and thrombin time (TT) were prolonged (P<0.05). Conclusion:Danshen Decoction could decrease whole blood viscosity to improve the blood stasis due to qi deficiency through improving the aggregation of platelet, inhibiting coagulation and the hyper-active release of platelet.

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