| 引用本文:王玉婷1，徐红日2，赵世同1，程淼1，姚兴伟3，常桂娇3，陈师林1，王成祥4.扶正解毒化瘀方对多重耐药铜绿假单胞菌MexAB-OprM外排泵表达影响的研究[J].世界中医药,2019,(04):. |
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| 扶正解毒化瘀方对多重耐药铜绿假单胞菌MexAB-OprM外排泵表达影响的研究 |
| Effects of Fuzheng Jiedu Huayu Decoction on the Expression of Efflux Pump MexAB-OprM of Multidrug-resistant Pseudomonas Aeruginosa |
| 投稿时间：2018-06-28 |
| DOI：10.3969/j.issn.1673-7202.2019.04.014 |
| 中文关键词: 扶正解毒化瘀方 多重耐药铜绿假单胞菌 外排泵 MexAB-OprM 最小抑菌浓度 生长曲线 实时荧光定量PCR 作用机制 |
| English Keywords:Fuzheng Jiedu Huayu Decoction Pseudomonas aeruginosa Efflux pump MexAB-OprM Minimum inhibitory concentration Growth curve Real-time fluorescence quantitative PCR Mechanism of action |
| 基金项目:国家自然科学基金面上项目（81573924） |
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| 中文摘要: |
| 目的：研究扶正解毒化瘀方对多重耐药铜绿假单胞菌MexAB-OprM外排泵表达的影响。方法：针对筛选出的MexAB-OprM阳性的铜绿假单胞菌菌株，通过微量稀释法，测定哌拉西林-他唑巴坦的最小抑菌浓度和扶正解毒化瘀方水提物的最小杀菌浓度，从而确定荧光定量PCR细菌样本时培养剂中哌拉西林-他唑巴坦和扶正解毒化瘀方水提物的浓度。绘制细菌生长曲线，确定细菌样本的培养时间。利用实时荧光定量PCR技术检测药物干预前后mexB、oprM、mexR的表达量。结果：扶正解毒化瘀方水提物能降低MexAB-OprM外排泵阳性MDRPA菌株的mexB、oprM表达量（P<0.05），同时能增加mexR的表达量（P<0.05）。结论：扶正解毒化瘀方对MDRPA所致肺炎的作用机制之一可能是通过抑制MexAB-OprM外排泵的表达实现的。 |
| English Summary: |
| To study the effects of Fuzheng Jiedu Huayu Decoction on the expression of MexAB-OprM efflux pumps in multidrug-resistant Pseudomonas aeruginosa.Methods:The minimum inhibitory concentration of piperacillin-tazobactam and the minimum bactericidal concentration of the water extract of Fuzheng Jiedu Huayu Decoction were determined via microdilution method for selected MexAB-OprM positive Pseudomonas aeruginosa strains so as to determine the concentration of piperacillin-tazobactam and the water extract of Fuzheng Jiedu Huayu Decoction in the culture medium during the fluorescence quantitative PCR of bacterial samples.The bacterial growth curve was drawn and the culture time of the bacterial samples was determined.The expression levels of mexB，oprM，and mexR before and after drug intervention were detected by real-time fluorescence quantitative PCR technology.Results:The expression levels of mexB and oprM in MexB-OprM efflux pump positive strains were decreased after the intervention of Fuzheng Jiedu Huayu Decoction water extract(P<0.05)，and that of mexR was increased than before(P<0.05).Conclusion:The therapeutic effect of Fuzheng Jiedu Huayu Decoction on pneumonia caused by MDRPA may be attributed to the inhibition of the expression of MexAB-OprM efflux pump. |
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