| 引用本文:吉晶1，2，何立群1，2.大黄素对TGF β1诱导的NRK 49F细胞增殖的影响[J].世界中医药,2019,(05):. |
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| 大黄素对TGF β1诱导的NRK 49F细胞增殖的影响 |
| Effects of Emodin on Proliferation of NRK-49F Cells Induced by TGF-β1 |
| 投稿时间：2019-04-10 |
| DOI：10.3969/j.issn.1673-7202.2019.05.007 |
| 中文关键词: 大黄素 NRK-49F细胞 纤连蛋白(FN) I型胶原蛋白(Col-I) Smad2/3 |
| English Keywords:Emodin Renal fibroblasts(NRK-49F) Fibronectin(FN) Col-I Smad2/3 |
| 基金项目:上海市名老中医学术经验研究工作室(SHGZS-2017027);上海中医药大学研究生“创新能力培养”专项科研项目(Y201804) |
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| 中文摘要: |
| 目的:研究大黄素对TGF-β1诱导的肾成纤维细胞株(NRK-49F)增殖以及降低FN(纤连蛋白),Col-I(I型胶原蛋白),Smad2/3蛋白及基因表达的机制。方法:采用CCK8法检测不同浓度大黄素对NRK-49F细胞增殖情况的影响;终浓度为20、40、80 μmol/L的大黄素处理NRK-49F细胞30 min后,模型组和观察组均以TGF-β1以5 ng/mL的终浓度刺激24 h,并收集细胞,蛋白质免疫印迹技术(Western blot)检测FN,Col-I,Smad2/3蛋白表达水平,实时荧光定量PCR(Real-time PCR)检测FN,Col-I,Smad2/3 mRNA表达。结果:模型组FN,Col-I,Smad2/3蛋白和基因的表达明显增加,大黄素含药血清组能抑制NRK-49F细胞增殖,且抑制FN,Col-I,Smad2/3蛋白和的表达(P<0.05)。大黄素浓度越高,抑制作用越明显,成一定的量效关系。结论:大黄素干预后,可改善肾纤维化程度,保护NRK-49F细胞模型损伤。 |
| English Summary: |
| To investigate the mechanism of emodin on the proliferation of TGF-β1 induced renal fibroblasts(NRK-49F)and the reduction of protein and gene expressions of FN(fibronectin),Col-I(typeⅠcollagen)and Smad2/3.Methods:The effects of different concentrations of emodin on the proliferation of NRK-49F cells were detected by CCK8 assay.NRK-49F cells were treated with emodin at a final concentrations of 20,40,80 μmol/L for 30 min.Both the model group and the treatment group were stimulated with TGF-β1 at a final concentration of 5 ng/mL for 24 h,and the cells were collected.The expression levels of FN,Col-I and Smad2/3 protein were detected by Western blot,and the expressions of FN,Col-I and Smad2/3 mRNA were detected by real-time PCR.Results:The expression of FN,Col-I,Smad2/3 protein and gene in the model group was significantly increased.The emodin-containing serum group inhibited the proliferation of NRK-49F cells and inhibited the expression of FN,Col-I,Smad2/3 protein(P<0.05).The higher the concentration of emodin was,the more obvious the inhibition was.Emodin concentration and inhibition were dose-effect relationships.Conclusion:After emodin intervention,it can improve the degree of renal fibrosis and protect the NRK-49F cell model injury. |
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