| 引用本文:邱灵慧，王铁山，刘碧原，臧颖颖，陈昕力，刘玥婷，陈慧昀，王朝阳，卢涛.刮痧力度及时间间隔对刮痧效果的影响[J].世界中医药,2019,(05):. |
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| 刮痧力度及时间间隔对刮痧效果的影响 |
| Effects of Intensity and Time Interval on the Efficacy of Gua Sha |
| 投稿时间：2018-10-18 |
| DOI：10.3969/j.issn.1673-7202.2019.05.016 |
| 中文关键词: 刮痧 力度 时间 免疫 组织切片 炎性反应因子 血常规 T淋巴细胞亚群 |
| English Keywords:Gua Sha Intensity Time interval Immunity Tissue sections Inflammatory factors Blood routine examination Tlymphocyte subpopulation |
| 基金项目:国家自然科学基金项目(81774110）；北京市自然科学基金项目（7162099） |
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| 中文摘要: |
| 目的：观察不同力度对刮痧效果的影响，初步探讨刮痧的作用机制以及效应周期，进一步探究刮痧治疗的最佳间隔时间。方法：用自制的特殊刮痧板控制使刮痧频次等参数保持一致，分别以1N、3N和9N的力进行刮痧，通过ELISA方法检测刮痧后IL-1α、IL-6的变化，结合皮肤HE染色法探究不同力度刮痧对机体的影响，选择适合的刮痧力度，通过血常规和流式细胞术检测刮痧后不同时间点的白细胞总数、淋巴细胞总数及T细胞亚群等的变化，初步探讨刮痧的作用机制和效应周期。结果：1N的力度刮痧未引起明显的变化，9N的力度刮痧造成了较严重的皮肤损伤和大量炎性反应因子释放，3N的力度刮痧较为适中，引起了机体适度的应激反应。以3N的力度刮痧后，血液中白细胞总数和淋巴细胞总数及IL-1α、IL-6缓慢升高，在1 h到24 h之间达到峰值，之后缓慢下降，在72 h左右恢复正常水平。结论：3N的力比较适合于刮痧实验，在刮痧之后1 h到24 h，刮痧对免疫系统的增强作用达到峰值，在72 h左右回归正常值，初步提示可以开始下一次刮痧治疗。 |
| English Summary: |
| To explore the effects of different intensity on the efficacy of Gua Sha，the working mechanism and the effective period of Gua Sha preliminarily，and to further explore the best time interval between two Gua Sha treatments.Methods:The parameters such as the frequency of Gua Sha were controlled by the self-made special Gua Sha plate，and Gua Sha was respectively performed with the force of 1N，3N and 9N.The changes of IL-1α and IL-6 after Gua Sha were detected by ELISA.The effects of Gua Sha with different intensity on the body was tested via the method of skin HE staining，and the appropriate Gua Sha intensity was selected.The changes of leukocyte count，lymphocyte count and T cell subpopulations at different time points after Gua Sha were detected by routine blood examination and flow cytometry.The working mechanism and effective period of Gua Sha were preliminarily discussed.Results:The Gua Sha of 1N intensity did not cause obvious changes，while Gua Sha of 9N intensity caused relatively serious skin injury and the release of a large number of inflammatory factors.Gua Sha of 3N intensity was of moderation，which caused moderate stress reaction of the body.After Gua Sha with 3N intensity，the total number of leukocytes and lymphocytes，IL-1α and IL-6 increased slowly，reached the peak between 1 hour and 24 hours，then decreased slowly，and returned to normal level at 72 h.Conclusion:3N intensity is suitable for Gua Sha experiments.In between 1 hour and 24 hours after Gua Sha，the boosting effect of Gua Sha on the immune system peaked，which returned normal at 72 hours after the performance，suggesting the proceeding of the next treatment. |
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