| 引用本文:林瑶，左滢竹，哈略，赵百孝.艾灸对快速老化小鼠海马区突触可塑性相关蛋白表达的影响〖JZ)〗〖HS)〗[J].世界中医药,2019,(05):. |
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| 艾灸对快速老化小鼠海马区突触可塑性相关蛋白表达的影响〖JZ)〗〖HS)〗 |
| Effects of Moxibustion on Expression of Synaptic Plasticity-related Proteins in Hippocampus of Senescence Accelerated Mouse Prone 8 |
| 投稿时间：2018-11-07 |
| DOI：10.3969/j.issn.1673-7202.2019.05.018 |
| 中文关键词: 阿尔茨海默病 艾灸 BDNF/Trk B通路 快速老化小鼠 脑源性神经营养因子 突触 突触可塑性 突触素 |
| English Keywords:Alzheimer's disease Moxibustion BDNF/Trk B pathway Senescence accelerated mouse prone 8 Brain-derived neurotrophic factor Synapse Synaptic plasticity Synaptophysin |
| 基金项目:国家自然科学基金项目(81574068) |
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| 中文摘要: |
| 目的:探究艾灸对快速老化小鼠(SAMP8)海马区突触可塑性相关蛋白表达的影响。方法:将12只6个月龄雄性SAMP8小鼠随机分为模型组和艾灸组。另以6只同月龄雄性正常老化小鼠(SAMR1)作为空白组。空白组、模型组小鼠每天常规抓取并用固定器固定,不予其他处理;艾灸组小鼠釆用相同抓取并固定后,用艾条灸其关元穴。各组小鼠干预时间为20 min/d,6 d/周,共干预6周。6周后取材,用Western-blot法检测各组小鼠海马突触素(SYN)、脑源性神经营养因子(BDNF)、酪氨酸激酶受体B(Trk B)及磷酸化Trk B(p-Trk B)的表达。结果:与空白组比较,模型组小鼠海马内SYN、BDNF、p-Trk B表达显著下降(P<0.05)。与模型组比较,艾灸组小鼠海马内SYN、BDNF、p-Trk B表达显著上升(P<0.05)。3组小鼠海马Trk B表达水平差异无统计学意义(P>0.05)。结论:艾灸干预能够通过影响SAMP8小鼠海马区突触可塑性相关蛋白的表达起到抗衰老的作用,且该作用可能经由BDNF/Trk B通路实现。 |
| English Summary: |
| To explore the effects of moxibustion on the expression of synaptic plasticity-related proteins in hippocampus of senescence accelerated mouse prone 8 (SAMP8).Methods:A total of 12 6-month-old male SAMP8 mice were randomly divided into a model group and a moxibustion group.In addition，6 male SAMR1 mice of the same age were used as a blank group.The blank and the model group were routinely grasped daily and fixed with a fixator，and no other treatment was performed; the mice in the moxibustion group were treated the same grasping and fixation，and Guanyuan (CV4) was given moxibustion.The intervention time of each group was 20 min/day，6 d/week，and a total of 6 weeks of intervention.After 6 weeks，the expression of synaptophysin (SYN)，brain-derived neurotrophic factor (BDNF)，tyrosine kinase receptor B (Trk B) and phosphorylated Trk B (p-Trk B) in hippocampus of the each group of mice were detected by Western blot.Results:Compared with the blank group，the expression of SYN，BDNF and p-Trk B in the hippocampus of the model group were significantly decreased (P<0.05).Compared with the model group，the expression of SYN，BDNF and p-Trk B in the hippocampus of the moxibustion group were significantly increased (P<0.05).There was no significant difference in the expression of Trk B in hippocampus between the 3 groups (P>0.05).Conclusion:Moxibustion intervention can play an anti-aging role by affecting the expression of synaptic plasticity-related proteins in the hippocampus of SAMP8 mice，and this effect may be achieved through BDNF/Trk B pathway. |
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