| 引用本文:杨光磊1，王国强1，米佳1，王秀阁2.涤痰汤糖尿病大鼠认知功能障碍的保护作用及机制[J].世界中医药,2019,(05):. |
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| 涤痰汤糖尿病大鼠认知功能障碍的保护作用及机制 |
| Protective Effects and Mechanism of Ditan Decoction on Cognitive Dysfunction in Diabetic Rats |
| 投稿时间：2019-03-11 |
| DOI：10.3969/j.issn.1673-7202.2019.05.021 |
| 中文关键词: 糖尿病模型 学习记忆能力 涤痰汤 水迷宫 海马 凋亡 PI3K-AKT信号通路 神经保护 |
| English Keywords:Diabetic model Learning and memory ability Ditan decoction Water maze Hippocampal Apoptosis PI3K-AKT signaling pathway Neuroprotection |
| 基金项目:吉林省重点科技攻关项目(20160204010YY);长春中医药大学科研项目(FK201801) |
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| 中文摘要: |
| 目的:观察涤痰汤对糖尿病大鼠认知能力的影响,并探讨其对PI3K-AKT信号通路相关因子的影响。方法:将30只SD鼠纳入研究,随机分为正常对照组、模型组及涤痰汤组,其中模型组及涤痰汤组大鼠均接受糖尿病模型制备,涤痰汤组用0.88 g/mL涤痰汤药液灌胃,正常对照组及模型组用等量的生理盐水灌胃,3组均连续灌胃28 d。各组采用水迷宫评估大鼠学习记忆功能,Tunel检测神经元细胞调亡情况,HE染色法观察海马组织病理结构改变,Western blotting法检测各组海马组织PI3K、AKT、p-AKT、Bax及Bcl-2蛋白水平变化。结果:涤痰汤可明显改善糖尿病模型大鼠的学习记忆能力,抑制神经元凋亡率及Bax水平,增加海马区PI3K、p-AKT、Bcl-2蛋白表达。结论:涤痰汤可明显改善糖尿病模型大鼠的认知,其作用机制可能与介导PI3K/AKT信号通路有关。 |
| English Summary: |
| To observe the effects of Ditan decoction on cognitive ability of diabetic rats and to explore the effects of Ditan decoction on PI3K-AKT signaling pathway related factors.Methods:A total of 30 SD rats were randomly divided into a normal control group,a model group and a Ditan decoction group.tTe rats in the model group and the Ditan decoction group were all prepared by the diabetic model,and the rats in the Ditan decoction group were treated with 0.88 g/mL Ditan decoction by gavage.The normal control group and the model group were given the same amount of normal saline intragastrically,and the 3 groups were continuously intragastrically administered for 28 days.The learning and memory function of rats in each group was evaluated by water maze,and the apoptosis of neurons was detected by tunnel.The pathological changes of hippocampus were observed by HE staining,and the levels of PI3K,AKT,p-AKT,Bax and Bcl-2 protein in hippocampus of each group were detected by Western blotting.Results:Ditan decoction could significantly improve the learning and memory ability of diabetic rats,inhibited the neuronal apoptosis rate and the level of Bax,and increased the expression of PI3K,p-AKT and Bcl-2 protein in hippocampus.Conclusion:Ditan decoction can significantly improve the cognition of diabetic rats,and its mechanism may be related to the mediation of PI3K/AKT signaling pathway. |
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